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Structure paper

TitleThe cation-pi box is a specific phosphatidylcholine membrane targeting motif.
Journal, issue, pagesJ. Biol. Chem., Vol. 288, Page 14863-14873, Year 2013
Publish dateDec 4, 2012 (structure data deposition date)
AuthorsCheng, J. / Goldstein, R. / Gershenson, A. / Stec, B. / Roberts, M.F.
External linksJ. Biol. Chem. / PubMed:23576432
MethodsX-ray diffraction
Resolution1.65 - 2.2 Å
Structure data

PDB-4i8y:
Structure of the unliganded N254Y/H258Y mutant of the phosphatidylinositol-specific phospholipase C from S. aureus
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-4i90:
Structure of the N254Y/H258Y mutant of the phosphatidylinositol-specific phospholipase C from S. aureus bound to choline
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-4i9j:
Structure of the N254Y/H258Y mutant of the phosphatidylinositol-specific phospholipase C from S. aureus bound to diC4PC
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-4i9m:
Structure of the N254Y/H258Y mutant of the phosphatidylinositol-specific phospholipase C from Staphylococcus aureus bound to HEPES
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-4i9t:
Structure of the H258Y mutant of the phosphatidylinositol-specific phospholipase C from Staphylococcus aureus
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-CHT:
CHOLINE ION

ChemComp-XP5:
(4S,7R)-7-(heptanoyloxy)-4-hydroxy-N,N,N-trimethyl-10-oxo-3,5,9-trioxa-4-phosphahexadecan-1-aminium 4-oxide

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-SO4:
SULFATE ION

ChemComp-INS:
1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE / neurotransmitter, hormone*YM

ChemComp-PGE:
TRIETHYLENE GLYCOL

Source
  • staphylococcus aureus subsp. aureus (bacteria)
Keywordshydrolase / lyase / TIM barrel / phospholipase / choline binding / diC4PC binding

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