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Structure paper

TitleWatching the Bacteriophage N4 RNA Polymerase Transcription by Time-dependent Soak-trigger-freeze X-ray Crystallography.
Journal, issue, pagesJ. Biol. Chem., Vol. 288, Page 3305-3311, Year 2013
Publish dateMay 30, 2012 (structure data deposition date)
AuthorsBasu, R.S. / Murakami, K.S.
External linksJ. Biol. Chem. / PubMed:23235152
MethodsX-ray diffraction
Resolution1.997 - 2.47 Å
Structure data

PDB-4ff1:
N4 mini-vRNAP transcription initiation complex, 1 min after soaking GTP, ATP and Mn
Method: X-RAY DIFFRACTION / Resolution: 2.47 Å

PDB-4ff2:
N4 mini-vRNAP transcription initiation complex, 2 min after soaking GTP, ATP and Mn
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4ff3:
N4 mini-vRNAP transcription initiation complex, 3 min after soaking GTP, ATP and Mn
Method: X-RAY DIFFRACTION / Resolution: 1.997 Å

PDB-4ff4:
N4 mini-vRNAP transcription initiation complex, 4 min after soaking GTP, ATP and Mn
Method: X-RAY DIFFRACTION / Resolution: 2.026 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MN:
Unknown entry

ChemComp-5GP:
GUANOSINE-5'-MONOPHOSPHATE

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-POP:
PYROPHOSPHATE 2-

Source
  • enterobacteria phage n4 (virus)
KeywordsTRANSFERASE/DNA / RNA polymerase / DNA / TRANSFERASE-DNA complex

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