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Structure paper

TitleBiochemical and structural insights into the fidelity of bacillus stearothermophilus DNA polymerase
Journal, issue, pagesTo be Published
Publish dateFeb 19, 2012 (structure data deposition date)
AuthorsGan, J.H. / Abdur, R. / Liu, H.H. / Sheng, J. / Caton-Willians, J. / Soares, A.S. / Huang, Z.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.85 - 2.86 Å
Structure data

PDB-4dsi:
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus with duplex DNA, Se-dGTP and Calcium
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-4dsj:
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus with duplex DNA, dGTP and Calcium
Method: X-RAY DIFFRACTION / Resolution: 2.86 Å

PDB-4dsk:
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus with duplex DNA, PPi and Calcium
Method: X-RAY DIFFRACTION / Resolution: 2.18 Å

PDB-4dsl:
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus with duplex DNA and Calcium
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-4dwi:
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus with self complementary DNA, Se-dGTP and Calcium
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-DGP:
2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-DGT:
2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE

ChemComp-POP:
PYROPHOSPHATE 2-

ChemComp-9MG:
9-METHYLGUANINE

Source
  • geobacillus stearothermophilus (bacteria)
KeywordsTRANSFERASE/DNA / DNA polymerase / polymerization fidelity / calcium cation / purine selectivity / blunt end extension / base stacking / DNA synthesis / TRANSFERASE-DNA complex / pyrophosphorolysis

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