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Structure paper

TitleStructural Explanation for Allolactose (lac operon inducer) Synthesis by lacZ beta-Galactosidase and the Evolutionary Relationship between Allolactose synthesis and the lac Repressor
Journal, issue, pagesJ. Biol. Chem., Vol. 288, Page 12993-13005, Year 2013
Publish dateFeb 22, 2012 (structure data deposition date)
AuthorsWheatley, R.W. / Lo, S. / Jancewicz, L.J. / Dugdale, M.L. / Huber, R.E.
External linksJ. Biol. Chem. / PubMed:23486479
MethodsX-ray diffraction
Resolution2.2 - 2.3 Å
Structure data

PDB-4duw:
E. coli (lacZ) beta-galactosidase (G974A) in complex with allolactose
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-4dux:
E. coli (lacZ) beta-galactosidase (N460S) in complex with L-ribose
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

ChemComp-HOH:
WATER

ChemComp-0MK:
beta-L-ribopyranose

Source
  • escherichia coli (E. coli)
KeywordsHYDROLASE / bi-functional enzyme / allolactose synthesis / TIM BARREL (ALPHA/BETA BARREL) / JELLY-ROLL BARREL / IMMUNOGLOBULIN / BETA SUPERSANDWICH / BETA-GALACTOSIDASE / GLYCOSIDASE

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