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TitleThe Isomerase and Hydratase Reaction Mechanism of the Crotonase Active Site of the Multifunctional Enzyme (Type-1), as Deduced from Structures of Complexes with 3S-Hydroxy- Acyl-Coa.
Journal, issue, pagesFEBS J., Vol. 280, Page 3160-, Year 2013
Publish dateJul 28, 2011 (structure data deposition date)
AuthorsKasaragod, P. / Schmitz, W. / Kalervo Hiltunen, J. / Wierenga, R.K.
External linksFEBS J. / PubMed:23351063
MethodsX-ray diffraction
Resolution2.3 - 3.1 Å
Structure data

PDB-3zw8:
Crystal Structure Of Rat Peroxisomal Multifunctional Enzyme Type 1 (rpMFE1) In Apo Form
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-3zw9:
CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 1 (RPMFE1) COMPLEXED WITH (2S,3S)-3-HYDROXY-2-METHYLBUTANOYL-COA
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-3zwa:
CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 1 (RPMFE1) COMPLEXED WITH 3S-HYDROXY-HEXANOYL-COA
Method: X-RAY DIFFRACTION / Resolution: 2.47 Å

PDB-3zwb:
CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 1 (RPMFE1) COMPLEXED WITH 2TRANS-HEXENOYL-COA
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-3zwc:
CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 1 (RPMFE1) COMPLEXED WITH 3S-HYDROXY-DECANOYL-COA
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-T1G:
(2S,3S)-3-HYDROXY-2-METHYLBUTANOYL-COA

ChemComp-3H9:
(S)-3-Hydroxyhexanoyl-CoA

ChemComp-TC6:
(2E)-Hexenoyl-CoA

ChemComp-HSC:
(S)-3-HYDROXYDECANOYL-COA

Source
  • rattus norvegicus (Norway rat)
KeywordsOXIDOREDUCTASE / BETA OXIDATION PATHWAY / LIPID METABOLISM / LYASE / ISOMERASE / PEROXISOME / FATTY ACID METABOLISM

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