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TitleCrystal Structures of the Viral Protease Npro Imply Distinct Roles for the Catalytic Water in Catalysis
Journal, issue, pagesStructure, Vol. 21, Page 929-, Year 2013
Publish dateDec 12, 2012 (structure data deposition date)
AuthorsZogg, T. / Sponring, M. / Schindler, S. / Koll, M. / Schneider, R. / Brandstetter, H. / Auer, B.
External linksStructure / PubMed:23643950
MethodsX-ray diffraction
Resolution1.25 - 2.65 Å
Structure data

PDB-3zfn:
Crystal structure of product-like, processed N-terminal protease Npro
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-3zfo:
Crystal structure of substrate-like, unprocessed N-terminal protease Npro mutant S169P
Method: X-RAY DIFFRACTION / Resolution: 2.01 Å

PDB-3zfp:
Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag
Method: X-RAY DIFFRACTION / Resolution: 1.25 Å

PDB-3zfq:
Crystal structure of product-like, processed N-terminal protease Npro with mercury
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-3zfr:
Crystal structure of product-like, processed N-terminal protease Npro with iridium
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-3zft:
Crystal structure of product-like, processed N-terminal protease Npro at pH 3
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-3zfu:
Crystal structure of substrate-like, unprocessed N-terminal protease Npro mutant S169P with sulphate
Method: X-RAY DIFFRACTION / Resolution: 1.76 Å

Chemicals

ChemComp-SGM:
MONOTHIOGLYCEROL / 3-Mercaptopropane-1,2-diol

ChemComp-CL:
Unknown entry / Chloride

ChemComp-HOH:
WATER / Water

ChemComp-OH:
HYDROXIDE ION / Hydroxide

ChemComp-HG:
Unknown entry / Mercury (element)

ChemComp-IR3:
IRIDIUM (III) ION / Iridium

ChemComp-SO4:
SULFATE ION / Sulfate

Source
  • pestivirus strain d32/00_hobi
KeywordsHYDROLASE / AUTO-PROCESSING CYSTEINE PROTEASE / VIRAL PROTEASE / IN CIS- CLEAVAGE / HYDROXIDE-DEPENDENT CATALYSIS / AUTO-PROTEOLYSIS / IMMUNE MODULATION / HOST-PATHOGEN INTERACTION / CONVERGENT EVOLUTION / PESTIVIRUS

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