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Structure paper

TitleCrystal structure of streptavidin mutant with low immunogenicity.
Journal, issue, pagesJ. Biosci. Bioeng., Vol. 119, Page 642-647, Year 2015
Publish dateSep 5, 2014 (structure data deposition date)
AuthorsKawato, T. / Mizohata, E. / Meshizuka, T. / Doi, H. / Kawamura, T. / Matsumura, H. / Yumura, K. / Tsumoto, K. / Kodama, T. / Inoue, T. / Sugiyama, A.
External linksJ. Biosci. Bioeng. / PubMed:25434833
MethodsX-ray diffraction
Resolution1 - 1.3 Å
Structure data

PDB-3wyp:
Crystal structure of wild-type core streptavidin in complex with D-biotin/biotin-D-sulfoxide at 1.3 A resolution
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-3wyq:
Crystal structure of the low-immunogenic core streptavidin mutant LISA-314 (Y22S/Y83S/R84K/E101D/R103K/E116N) at 1.0 A resolution
Method: X-RAY DIFFRACTION / Resolution: 1 Å

Chemicals

ChemComp-BTN:
BIOTIN

ChemComp-GOL:
GLYCEROL

ChemComp-BSO:
BIOTIN-D-SULFOXIDE

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

Source
  • streptomyces avidinii (bacteria)
KeywordsBIOTIN BINDING PROTEIN / beta-barrel

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