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Structure paper

TitleFlavin-mediated dual oxidation controls an enzymatic Favorskii-type rearrangement.
Journal, issue, pagesNature, Vol. 503, Page 552-556, Year 2013
Publish dateMar 22, 2013 (structure data deposition date)
AuthorsTeufel, R. / Miyanaga, A. / Michaudel, Q. / Stull, F. / Louie, G. / Noel, J.P. / Baran, P.S. / Palfey, B. / Moore, B.S.
External linksNature / PubMed:24162851
MethodsX-ray diffraction
Resolution1.67 - 1.95 Å
Structure data

PDB-3w8w:
The crystal structure of EncM
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-3w8x:
The complex structure of EncM with trifluorotriketide
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

PDB-3w8z:
The complex structure of EncM with hydroxytetraketide
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-4xlo:
Crystal Structure of EncM (crystallized with 4 mM NADPH)
Method: X-RAY DIFFRACTION / Resolution: 1.67 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-HOH:
WATER

ChemComp-FTK:
6,6,6-trifluoro-1-phenylhexane-1,3,5-trione

ChemComp-GOL:
GLYCEROL

ChemComp-HTK:
(7S)-7-hydroxy-1-phenyloctane-1,3,5-trione

Source
  • streptomyces maritimus (bacteria)
KeywordsOXIDOREDUCTASE / monooxygenase / flavin binding / Flavoenzyme / NADPH / vanillyl-alcohol oxidase/p-cresol methylhydroxylase fold / oxygenase

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