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Structure paper

TitleX-ray crystal structure of a mutant assimilatory nitrite reductase that shows sulfite reductase-like activity
Journal, issue, pagesChem. Biodivers., Vol. 9, Page 1989-1999, Year 2012
Publish dateDec 5, 2011 (structure data deposition date)
AuthorsNakano, S. / Takahashi, M. / Sakamoto, A. / Morikawa, H. / Katayanagi, K.
External linksChem. Biodivers. / PubMed:22976986
MethodsX-ray diffraction
Resolution1.35 - 2.07 Å
Structure data

PDB-3vlx:
Assimilatory nitrite reductase (Nii3) - N226K mutant - ligand free form from tobacco leaf
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-3vly:
Assimilatory nitrite reductase (Nii3) - N226K mutant - SO3 partial complex from tobacco leaf
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

PDB-3vlz:
Assimilatory nitrite reductase (Nii3) - N226K mutant - SO3 full complex from tobacco leaf
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

PDB-3vm0:
Assimilatory nitrite reductase (Nii3) - N226K mutant - NO2 complex from tobacco leaf
Method: X-RAY DIFFRACTION / Resolution: 1.695 Å

PDB-3vm1:
assimilatory nitrite reductase (Nii3) - N226K mutant - HCO3 complex from tobacco leaf
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-SRM:
SIROHEME

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-K:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-SO3:
SULFITE ION

ChemComp-NO2:
NITRITE ION

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

Source
  • nicotiana tabacum (common tobacco)
KeywordsOXIDOREDUCTASE / Nii3 N226K mutant / ligand free / 3 alpha/beta domains / reductase / SO3 partial complex / SO3 full complex / NO2 complex / HCO3 complex

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