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Structure paper

TitleInsights into the mechanism of the antibiotic-synthesizing enzyme MoeO5 from crystal structures of different complexes
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 51, Page 4157-4160, Year 2012
Publish dateNov 8, 2011 (structure data deposition date)
AuthorsRen, F. / Ko, T.-P. / Feng, X. / Huang, C.-H. / Chan, H.-C. / Hu, Y. / Wang, K. / Ma, Y. / Liang, P.-H. / Wang, A.H.-J. ...Ren, F. / Ko, T.-P. / Feng, X. / Huang, C.-H. / Chan, H.-C. / Hu, Y. / Wang, K. / Ma, Y. / Liang, P.-H. / Wang, A.H.-J. / Oldfield, E. / Guo, R.-T.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:22431288
MethodsX-ray diffraction
Resolution1.39 - 1.8 Å
Structure data

PDB-3vk5:
Crystal structure of MoeO5 in complex with its product FPG
Method: X-RAY DIFFRACTION / Resolution: 1.39 Å

PDB-3vka:
Crystal structure of MoeO5 soaked for 3 hours in FsPP
Method: X-RAY DIFFRACTION / Resolution: 1.57 Å

PDB-3vkb:
Crystal structure of MoeO5 soaked with FsPP overnight
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-3vkc:
Crystal structure of MoeO5 soaked with pyrophosphate
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

PDB-3vkd:
Crystal structure of MoeO5 soaked with 3-phosphoglycerate
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

Chemicals

ChemComp-FPQ:
(2R)-3-(phosphonooxy)-2-{[(2Z,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy}propanoic acid

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

ChemComp-POP:
PYROPHOSPHATE 2-

ChemComp-FPS:
S-[(2E,6E)-3,7,11-TRIMETHYLDODECA-2,6,10-TRIENYL] TRIHYDROGEN THIODIPHOSPHATE

ChemComp-PO4:
PHOSPHATE ION

Source
  • streptomyces ghanaensis (bacteria)
KeywordsTRANSFERASE / TIM barrel

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