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Structure paper

TitleStereospecific Formation of a Ternary Complex of (S)-alpha, beta-Fluoromethylene-dATP with DNA Pol beta.
Journal, issue, pagesChembiochem, Vol. 13, Page 528-530, Year 2012
Publish dateAug 16, 2011 (structure data deposition date)
AuthorsChamberlain, B.T. / Batra, V.K. / Beard, W.A. / Kadina, A.P. / Shock, D.D. / Kashemirov, B.A. / McKenna, C.E. / Goodman, M.F. / Wilson, S.H.
External linksChembiochem / PubMed:22315190
MethodsX-ray diffraction
Resolution2 Å
Structure data

PDB-3tfr:
Ternary complex structure of DNA polymerase beta with a gapped DNA substrate and a, b dAMP(CF2)PP in the active site
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3tfs:
Ternary complex structure of DNA polymerase beta with a gapped DNA substrate and a, b dAMP(CFH)PP in the active site: Stereoselective binding of (S) isomer
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-F3A:
2'-deoxy-5'-O-[(S)-{difluoro[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}(hydroxy)phosphoryl]adenosine

ChemComp-MG:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-FHA:
2'-deoxy-5'-O-[(S)-{(S)-fluoro[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}(hydroxy)phosphoryl]adenosine

Source
  • homo sapiens (human)
KeywordsTRANSFERASE/DNA / DNA Polymerase / stereoselectivity / TRANSFERASE-DNA complex

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