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Structure paper

TitleKlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry.
Journal, issue, pagesNat. Chem. Biol., Vol. 8, Page 612-614, Year 2012
Publish dateMay 4, 2011 (structure data deposition date)
AuthorsBetz, K. / Malyshev, D.A. / Lavergne, T. / Welte, W. / Diederichs, K. / Dwyer, T.J. / Ordoukhanian, P. / Romesberg, F.E. / Marx, A.
External linksNat. Chem. Biol. / PubMed:22660438
MethodsX-ray diffraction
Resolution1.9 - 2.15 Å
Structure data

PDB-3rtv:
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a closed ternary complex with natural primer/template DNA
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-3sv3:
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a closed ternary complex with the artificial base pair dNaM-d5SICSTP
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-3sv4:
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in an open binary complex with dT as templating nucleobase
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

PDB-3syz:
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in an open binary complex with dNaM as templating nucleobase
Method: X-RAY DIFFRACTION / Resolution: 1.952 Å

PDB-3sz2:
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in an open binary complex with dG as templating nucleobase
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

Chemicals

ChemComp-DCP:
2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-5SI:
2-{2-deoxy-5-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-erythro-pentofuranosyl}-6-methylisoquinoline-1(2H)-thione

ChemComp-FMT:
FORMIC ACID

Source
  • thermus aquaticus (bacteria)
KeywordsTRANSFERASE/DNA / DNA polymerase / TRANSFERASE-DNA complex / artificial base pair / artificial base / binary complex

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