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Structure paper

TitleStructural basis for the synthesis of nucleobase modified DNA by Thermus aquaticus DNA polymerase.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 107, Page 21327-21331, Year 2010
Publish dateAug 23, 2010 (structure data deposition date)
AuthorsObeid, S. / Baccaro, A. / Welte, W. / Diederichs, K. / Marx, A.
External linksProc. Natl. Acad. Sci. USA / PubMed:21123743
MethodsX-ray diffraction
Resolution1.9 - 2 Å
Structure data

PDB-3ojs:
Snapshots of the large fragment of DNA polymerase I from Thermus Aquaticus processing C5 modified thymidines
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-3oju:
Snapshot of the large fragment of DNA polymerase I from Thermus Aquaticus processing c5 modified thymidies
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-XJS:
2'-deoxy-5-[9-(3-{[4-(diethylamino)-4-oxobutanoyl]amino}propyl)-18-ethyl-5,8,14,17-tetraoxo-4,9,13,18-tetraazaicos-1-yn-1-yl]uridine 5'-(tetrahydrogen triphosphate)

ChemComp-GOL:
GLYCEROL

ChemComp-MG:
Unknown entry

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-SSJ:
2'-deoxy-5-[(1-hydroxy-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)ethynyl]uridine 5'-(tetrahydrogen triphosphate)

Source
  • thermus aquaticus (bacteria)
KeywordsTransferase/DNA / DNA polymerase / C5 modified nucleotide analogs / binding pocket / denron-labeled triphosphate / Transferase-DNA complex / spin-labeled triphosphate

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