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Structure paper

TitleStructural investigation of myo-inositol dehydrogenase from Bacillus subtilis: implications for catalytic mechanism and inositol dehydrogenase subfamily classification.
Journal, issue, pagesBiochem. J., Vol. 432, Page 237-247, Year 2010
Publish dateMay 11, 2010 (structure data deposition date)
Authorsvan Straaten, K.E. / Zheng, H. / Palmer, D.R. / Sanders, D.A.
External linksBiochem. J. / PubMed:20809899
MethodsX-ray diffraction
Resolution1.539 - 2.9006 Å
Structure data

PDB-3mz0:
Crystal structure of apo myo-inositol dehydrogenase from Bacillus subtilis
Method: X-RAY DIFFRACTION / Resolution: 1.539 Å

PDB-3nt2:
Crystal structure of myo-inositol dehydrogenase from Bacillus subtilis with bound cofactor
Method: X-RAY DIFFRACTION / Resolution: 2.3003 Å

PDB-3nt4:
Crystal structure of myo-inositol dehydrogenase from Bacillus subtilis with bound cofactor NADH and inositol
Method: X-RAY DIFFRACTION / Resolution: 2.5001 Å

PDB-3nt5:
Crystal structure of myo-inositol dehydrogenase from Bacillus subtilis with bound cofactor and product inosose
Method: X-RAY DIFFRACTION / Resolution: 2.9006 Å

PDB-3nto:
Crystal structure of K97V mutant myo-inositol dehydrogenase from Bacillus subtilis
Method: X-RAY DIFFRACTION / Resolution: 1.9124 Å

PDB-3ntq:
Crystal structure of K97V mutant myo-inositol dehydrogenase from Bacillus subtilis with bound cofactor NAD
Method: X-RAY DIFFRACTION / Resolution: 2.6004 Å

PDB-3ntr:
Crystal structure of K97V mutant of myo-inositol dehydrogenase from Bacillus subtilis with bound cofactor NAD and inositol
Method: X-RAY DIFFRACTION / Resolution: 2.6503 Å

Chemicals

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-GOL:
GLYCEROL

ChemComp-CL:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-NAI:
1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE

ChemComp-INS:
1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE

ChemComp-ISE:
(2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

Source
  • bacillus subtilis (bacteria)
KeywordsOXIDOREDUCTASE / myo-inositol dehydrogenase / BsIDH / N-terminal Rossmann fold domain / glyceraldehyde-3-phosphate like C-terminal domain / NAD / myo-inositol / cofactor NAD(H) / substrate inositol / NAD(H) / inositol / inosose / K97V mutant

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