Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
Journal, issue, pages	PLoS Biol, Vol. 8, Issue 9, Year 2010
Publish date	Sep 14, 2010
Authors	Natacha Opalka / Jesse Brown / William J Lane / Kelly-Anne F Twist / Robert Landick / Francisco J Asturias / Seth A Darst /
PubMed Abstract	The Escherichia coli transcription system is the best characterized from a biochemical and genetic point of view and has served as a model system. Nevertheless, a molecular understanding of the ...The Escherichia coli transcription system is the best characterized from a biochemical and genetic point of view and has served as a model system. Nevertheless, a molecular understanding of the details of E. coli transcription and its regulation, and therefore its full exploitation as a model system, has been hampered by the absence of high-resolution structural information on E. coli RNA polymerase (RNAP). We use a combination of approaches, including high-resolution X-ray crystallography, ab initio structural prediction, homology modeling, and single-particle cryo-electron microscopy, to generate complete atomic models of E. coli core RNAP and an E. coli RNAP ternary elongation complex. The detailed and comprehensive structural descriptions can be used to help interpret previous biochemical and genetic data in a new light and provide a structural framework for designing experiments to understand the function of the E. coli lineage-specific insertions and their role in the E. coli transcription program.
External links	PLoS Biol / PubMed:20856905 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	1.6 - 11.2 Å
Structure data	5169 3lu0 EMDB-5169: Single-particle cryo-EM reconstruction of E. coli core RNA polymerase PDB-3lu0: Molecular model of Escherichia coli core RNA polymerase Method: EM (single particle) / Resolution: 11.0 Å PDB-3lti: Crystal structure of the Escherichia coli RNA polymerase beta subunit beta2-betai4 domains Method: X-RAY DIFFRACTION / Resolution: 1.6 Å
Chemicals	ChemComp-HOH: WATER ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry
Source	escherichia coli (E. coli)
Keywords	TRANSFERASE / BBM2 / DNA-directed RNA polymerase / Nucleotidyltransferase / Transcription / E. coli RNA polymerase