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TitleCryo-EM structure of a molluscan hemocyanin suggests its allosteric mechanism.
Journal, issue, pagesStructure, Vol. 21, Issue 4, Page 604-613, Year 2013
Publish dateApr 2, 2013
AuthorsQinfen Zhang / Xinghong Dai / Yao Cong / Junjie Zhang / Dong-Hua Chen / Matthew T Dougherty / Jiangyong Wang / Steven J Ludtke / Michael F Schmid / Wah Chiu /
PubMed AbstractHemocyanins are responsible for transporting O2 in the arthropod and molluscan hemolymph. Haliotis diversicolor molluscan hemocyanin isoform 1 (HdH1) is an 8 MDa oligomer. Each subunit is made up of ...Hemocyanins are responsible for transporting O2 in the arthropod and molluscan hemolymph. Haliotis diversicolor molluscan hemocyanin isoform 1 (HdH1) is an 8 MDa oligomer. Each subunit is made up of eight functional units (FUs). Each FU contains two Cu ions, which can reversibly bind an oxygen molecule. Here, we report a 4.5 A° cryo-EM structure of HdH1. The structure clearly shows ten asymmetric units arranged with D5 symmetry. Each asymmetric unit contains two structurally distinct but chemically identical subunits. The map is sufficiently resolved to trace the entire subunit Ca backbone and to visualize densities corresponding to some large side chains, Cu ion pairs, and interaction networks of adjacent subunits. A FU topology path intertwining between the two subunits of the asymmetric unit is unambiguously determined. Our observations suggest a structural mechanism for the stability of the entire hemocyanin didecamer and 20 ‘‘communication clusters’’ across asymmetric units responsible for its allosteric property upon oxygen binding.
External linksStructure / PubMed:23541894 / PubMed Central
MethodsEM (single particle)
Resolution4.5 - 7.8 Å
Structure data

EMDB-1648:
Assembly and Allosteric Mechanism of Molluscan Hemocyanin Revealed by Cryo-EM Structure and Pseudo-atomic Model
Method: EM (single particle) / Resolution: 7.8 Å

EMDB-5585:
Electron cryo-microscopy of Haliotis diversicolor molluscan hemocyanin isoform 1 (HdH1)
Method: EM (single particle) / Resolution: 4.5 Å

5586

3j32

EMDB-5586: An asymmetric unit map from Electron cryo-microscopy of Haliotis diversicolor molluscan hemocyanin isoform 1 (HdH1)
PDB-3j32: An asymmetric unit map from electron cryo-microscopy of Haliotis diversicolor molluscan hemocyanin isoform 1 (HdH1)
Method: EM (single particle) / Resolution: 4.5 Å

Source
  • haliotis diversicolor (invertebrata)
KeywordsOXYGEN TRANSPORT / allosteric

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