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Structure paper

TitleStructural Basis of Glyphosate Resistance Resulting from the Double Mutation Thr97 -> Ile and Pro101 -> Ser in 5-Enolpyruvylshikimate-3-phosphate Synthase from Escherichia coli.
Journal, issue, pagesJ. Biol. Chem., Vol. 284, Page 9854-9860, Year 2009
Publish dateDec 15, 2008 (structure data deposition date)
AuthorsFunke, T. / Yang, Y. / Han, H. / Healy-Fried, M. / Olesen, S. / Becker, A. / Schonbrunn, E.
External linksJ. Biol. Chem. / PubMed:19211556
MethodsX-ray diffraction
Resolution1.7 - 1.75 Å
Structure data

PDB-3fjx:
E. coli EPSP synthase (T97I) liganded with S3P
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-3fjz:
E. coli EPSP synthase (T97I) liganded with S3P and glyphosate
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-3fk0:
E. coli EPSP synthase (TIPS mutation) liganded with S3P
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-3fk1:
E. coli EPSP synthase (TIPS mutation) liganded with S3P and glyphosate
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-S3P:
SHIKIMATE-3-PHOSPHATE

ChemComp-FMT:
FORMIC ACID

ChemComp-HOH:
WATER

ChemComp-SER:
SERINE

ChemComp-GPF:
N-(phosphonomethyl)glycine

Source
  • escherichia coli k-12 (bacteria)
  • escherichia coli (E. coli)
KeywordsTRANSFERASE / inside-out alpha-beta barrel / Amino-acid biosynthesis / Aromatic amino acid biosynthesis / Cytoplasm

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