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TitleEvolution of enzymatic activities in the enolase superfamily: stereochemically distinct mechanisms in two families of cis,cis-muconate lactonizing enzymes.
Journal, issue, pagesBiochemistry, Vol. 48, Page 1445-1453, Year 2009
Publish dateApr 11, 2008 (structure data deposition date)
AuthorsSakai, A. / Fedorov, A.A. / Fedorov, E.V. / Schnoes, A.M. / Glasner, M.E. / Brown, S. / Rutter, M.E. / Bain, K. / Chang, S. / Gheyi, T. ...Sakai, A. / Fedorov, A.A. / Fedorov, E.V. / Schnoes, A.M. / Glasner, M.E. / Brown, S. / Rutter, M.E. / Bain, K. / Chang, S. / Gheyi, T. / Sauder, J.M. / Burley, S.K. / Babbitt, P.C. / Almo, S.C. / Gerlt, J.A.
External linksBiochemistry / PubMed:19220063
MethodsX-ray diffraction
Resolution1.6 - 2 Å
Structure data

PDB-3ct2:
Crystal structure of muconate cycloisomerase from Pseudomonas fluorescens
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-3dg3:
Crystal structure of muconate lactonizing enzyme from Mucobacterium Smegmatis
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-3dg6:
Crystal structure of muconate lactonizing enzyme from Mucobacterium Smegmatis complexed with muconolactone
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-3dg7:
Crystal structure of muconate lactonizing enzyme from Mucobacterium Smegmatis complexed with muconolactone
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3dgb:
Crystal structure of muconate lactonizing enzyme from Pseudomonas Fluorescens complexed with muconolactone
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-3fj4:
Crystal structure of muconate lactonizing enzyme from Pseudomonas Fluorescens complexed with muconolactone
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MUC:
[(2S)-5-oxo-2,5-dihydrofuran-2-yl]acetic acid

Source
  • pseudomonas fluorescens (bacteria)
  • mycobacterium smegmatis (bacteria)
KeywordsISOMERASE / Structural genomics / target 9450f / MLE / PSI-2 / Protein Structure Initiative / New York SGX Research Center for Structural Genomics / NYSGXRC / muconate lactonizing enzyme / muconolactone binding / cis / cis-muconate

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