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TitleStructure-guided mutagenesis for the improvement of substrate specificity of Bacillus megaterium glucose 1-dehydrogenase IV
Journal, issue, pagesFebs J., Vol. 279, Page 3264-3275, Year 2012
Publish dateFeb 16, 2011 (structure data deposition date)
AuthorsNishioka, T. / Yasutake, Y. / Nishiya, Y. / Tamura, T.
External linksFebs J. / PubMed:22804868
MethodsX-ray diffraction
Resolution1.9 - 2.1 Å
Structure data

PDB-3aus:
Crystal structure of Bacillus megaterium glucose dehydrogenase 4 in ligand-free form
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3aut:
Crystal structure of Bacillus megaterium glucose dehydrogenase 4 in complex with NADH
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3auu:
Crystal structure of Bacillus megaterium glucose dehydrogenase 4 in complex with D-glucose
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3ay6:
Crystal structure of Bacillus megaterium glucose dehydrogenase 4 A258F mutant in complex with NADH and D-glucose
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-3ay7:
Crystal structure of Bacillus megaterium glucose dehydrogenase 4 G259A mutant
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-NAI:
1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE

ChemComp-BGC:
beta-D-glucopyranose

ChemComp-CL:
Unknown entry

Source
  • bacillus megaterium (bacteria)
KeywordsOXIDOREDUCTASE / Rossmann Fold / NAD / Oxidation-reduction process / Cytosol / Nucleotide binding

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