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TitleRegio- and stereospecificity of filipin hydroxylation sites revealed by crystal structures of cytochrome P450 105P1 and 105D6 from Streptomyces avermitilis
Journal, issue, pagesJ. Biol. Chem., Vol. 285, Page 16844-16853, Year 2010
Publish dateDec 4, 2009 (structure data deposition date)
AuthorsXu, L.H. / Fushinobu, S. / Takamatsu, S. / Wakagi, T. / Ikeda, H. / Shoun, H.
External linksJ. Biol. Chem. / PubMed:20375018
MethodsX-ray diffraction
Resolution1.8 - 2.3 Å
Structure data

PDB-3aba:
Crystal structure of CYP105P1 in complex with filipin I
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-3abb:
Crystal structure of CYP105D6
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-FLI:
(3R,4S,6S,8S,10R,12R,14R,16S,17E,19E,21E,23E,25E,28R)-3-hexyl-4,6,8,10,12,14,16-heptahydroxy-17,28-dimethyloxacyclooctacosa-17,19,21,23,25-pentaen-2-one

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

Source
  • streptomyces avermitilis (bacteria)
KeywordsOXIDOREDUCTASE/ANTIBIOTIC / P450 / oxidoreductase / heme / monooxygenase / macrolide / filipin / Iron / Metal-binding / OXIDOREDUCTASE-ANTIBIOTIC complex

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