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TitleChaperoning by dynamic encasement: Structural basis of linker histone H1 nuclear import.
Journal, issue, pagesStructure, Year 2026
Publish dateSep 25, 2026
AuthorsZhenglin Fu / Fatma Chafra / Bernard Freytag / Trevor Huyton / Dirk Görlich /
PubMed AbstractImportins transport numerous proteins into cell nuclei. They also chaperone highly positively charged cargoes against ionic aggregation during transit. An extreme example is histone H1, which ...Importins transport numerous proteins into cell nuclei. They also chaperone highly positively charged cargoes against ionic aggregation during transit. An extreme example is histone H1, which requires two importins, Importin 7 (Imp7) and Importin β (Impβ), for safe import. Here, we present a combined cryoelectron microscopy (cryo-EM)/molecular dynamics (MD) structure of the Imp7·Impβ·H1 complex, correcting a previous model (PDB 6N88) with misassigned importin chains. Our structure reveals that Imp7 contacts Impβ via three interfaces and that this heterodimerization creates a large negatively charged cavity. The globular H1-domain is recognized by Imp7 alone. The highly cationic C-terminal H1-tail participates in fluid-like transient interactions, contacting the inner surface of Impβ before returning to the α-solenoid and long acidic loop of Imp7. This architecture and dynamics explain how the importins shield the histone's enormous positive charge while avoiding interactions too strong for efficient intranuclear cargo release. Finally, we report a RanGTP·Imp7 structure, illustrating how RanGTP dissociates the import complex inside nuclei.
External linksStructure / PubMed:42790418
MethodsEM (single particle)
Resolution3.6 Å
Structure data

57539

30fm

30hd

EMDB-57539: Structure of histone H1 in an import-chaperone complex with importin beta and importin 7
PDB-30fm: Structure of histone H1 in an import-chaperone complex with importin beta and importin 7 (full-length model)
PDB-30hd: Structure of histone H1 in an import-chaperone complex with importin beta and importin 7 (ordered regions)
Method: EM (single particle) / Resolution: 3.6 Å

57835

30jz

EMDB-57835, PDB-30jz:
Structure of Importin 7 in complex with RanGTP
Method: EM (single particle) / Resolution: 3.6 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • xenopus laevis (African clawed frog)
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN / Importin 7 / histone H1 / Importin beta / RanGTP

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