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Structure paper

TitleClosed complex of the D-3-hydroxybutyrate dehydrogenase induced by an enantiomeric competitive inhibitor.
Journal, issue, pagesJ. Biochem., Vol. 145, Page 467-479, Year 2009
Publish dateOct 7, 2008 (structure data deposition date)
AuthorsNakashima, K. / Ito, K. / Nakajima, Y. / Yamazawa, R. / Miyakawa, S. / Yoshimoto, T.
External linksJ. Biochem. / PubMed:19122202
MethodsX-ray diffraction
Resolution1.8 - 2.3 Å
Structure data

PDB-2ztl:
Closed conformation of D-3-hydroxybutyrate dehydrogenase complexed with NAD+ and L-3-hydroxybutyrate
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-2ztm:
T190S mutant of D-3-hydroxybutyrate dehydrogenase
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-2ztu:
T190A mutant of D-3-hydroxybutyrate dehydrogenase complexed with NAD+
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-2ztv:
The binary complex of D-3-hydroxybutyrate dehydrogenase with NAD+
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-3HL:
(3S)-3-HYDROXYBUTANOIC ACID

ChemComp-MG:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • pseudomonas fragi (bacteria)
KeywordsOXIDOREDUCTASE / short chain dehydrogenase/reductase / SDR family / NAD / NADH / HBDH / short chain dehydrogenase redactase

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