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Structure paper

TitleCrystallographic Snapshots of Tyrosine Phenol-Lyase Show that Substrate Strain Plays a Role in C-C Bond Cleavage
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 133, Page 16468-, Year 2011
Publish dateMar 16, 2011 (structure data deposition date)
AuthorsMilic, D. / Demidkina, T.V. / Faleev, N.G. / Phillips, R.S. / Matkovic-Calogovic, D. / Antson, A.A.
External linksJ. Am. Chem. Soc. / PubMed:21899319
MethodsX-ray diffraction
Resolution2 - 2.25 Å
Structure data

PDB-2ycn:
Y71F mutant of tyrosine phenol-lyase from Citrobacter freundii in complex with quinonoid intermediate formed with 3-fluoro-L-tyrosine
Method: X-RAY DIFFRACTION / Resolution: 2.04 Å

PDB-2ycp:
F448H mutant of tyrosine phenol-lyase from Citrobacter freundii in complex with quinonoid intermediate formed with 3-fluoro-L-tyrosine
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-2yct:
Tyrosine phenol-lyase from Citrobacter freundii in complex with pyridine N-oxide and the quinonoid intermediate formed with L-alanine
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-P61:
(2E)-3-(3-fluoro-4-hydroxyphenyl)-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}propanoic acid

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-P33:
3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL / precipitant*YM

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE

ChemComp-PLI:
(2E)-2-{[(Z)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4(1H)-YLIDENE}METHYL]IMINO}PROPANOIC ACID

ChemComp-9PO:
PYRIDINE-N-OXIDE

ChemComp-PO4:
PHOSPHATE ION

Source
  • citrobacter freundii (bacteria)
KeywordsLYASE / PYRIDOXAL 5'-PHOSPHATE DEPENDENT ENZYME / BETA-ELIMINATION

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