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Structure paper

TitleReversible Bond Formation Enables the Replication and Amplification of a Crosslinking Salen Complex as an Orthogonal Base Pair.
Journal, issue, pagesNature Chem., Vol. 3, Page 794-, Year 2011
Publish dateNov 15, 2010 (structure data deposition date)
AuthorsKaul, C. / Mueller, M. / Wagner, M. / Schneider, S. / Carell, T.
External linksNature Chem. / PubMed:21941252
MethodsX-ray diffraction
Resolution2.22 - 3.05 Å
Structure data

PDB-2xy5:
Crystal structure of an artificial salen-copper basepair in complex with fragment DNA polymerase I from Bacillus stearothermophilus
Method: X-RAY DIFFRACTION / Resolution: 2.22 Å

PDB-2xy6:
Crystal structure of a salicylic aldehyde basepair in complex with fragment DNA polymerase I from Bacillus stearothermophilus
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-2xy7:
Crystal structure of a salicylic aldehyde base in the pre-insertion site of fragment DNA polymerase I from Bacillus stearothermophilus
Method: X-RAY DIFFRACTION / Resolution: 3.05 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM

ChemComp-CU1:
COPPER (I) ION

ChemComp-EDN:
ETHANE-1,2-DIAMINE

ChemComp-HOH:
WATER

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

Source
  • geobacillus stearothermophilus (bacteria)
  • synthetic construct (others)
KeywordsTRANSFERASE/DNA / TRANSFERASE-DNA COMPLEX / SYNTHETIC BIOLOGY / METAL BASEPAIR / REPLICATION / SALEN COMPLEX

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