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| Title | 4.6A Cryo-EM reconstruction of tobacco mosaic virus from images recorded at 300 keV on a 4k x 4k CCD camera. |
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| Journal, issue, pages | J Struct Biol, Vol. 171, Issue 3, Page 303-308, Year 2010 |
| Publish date | Jun 15, 2010 |
 Authors | Daniel K Clare / Elena V Orlova /  |
| PubMed Abstract | Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a ...Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a helical form with RNA (X-ray fibre diffraction) and as a second distinct helical form with RNA (cryo-EM). Here we present a structural analysis of TMV as a test object to assess the quality of cryo-EM images recorded at 300 keV on a CCD camera. The 4.6A TMV structure obtained is consistent with the previous cryo-EM structure and confirms that there is a second helical form of TMV. The structure here also shows that with a similar number of TMV segments an equivalent resolution can be achieved with a 4k CCD camera at 300 keV. |
 External links | J Struct Biol / PubMed:20558300 / PubMed Central |
| Methods | EM (helical sym.) |
| Resolution | 4.6 Å |
| Structure data | |
| Source |
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 Keywords | VIRUS / SINGLE PARTICLE PROCESSING |
