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TitleConformational Changes Induced by Binding Udp-2F-Galactose to Alpha-1,3 Galactosyltransferase-Implications for Catalysis.
Journal, issue, pagesJ. Mol. Biol., Vol. 369, Page 1270-, Year 2007
Publish dateDec 25, 2006 (structure data deposition date)
AuthorsJamaluddin, H. / Tumbale, P. / Withers, S.G. / Acharya, K.R. / Brew, K.
External linksJ. Mol. Biol. / PubMed:17493636
MethodsX-ray diffraction
Resolution1.8 - 3.29 Å
Structure data

PDB-2jcj:
Crystal structure of alpha-1,3 Galactosyltransferase (C-terminus truncated mutant-C3) in complex with UDP and Tris
Method: X-RAY DIFFRACTION / Resolution: 2.02 Å

PDB-2jck:
Crystal structure of alpha-1,3 Galactosyltransferase (R365K) in complex with UDP and 2 manganese ion
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-2jcl:
Crystal structure of alpha-1,3 Galactosyltransferase (R365K) in the absence of ligands
Method: X-RAY DIFFRACTION / Resolution: 3.29 Å

PDB-2jco:
Crystal structure of wild type alpha-1,3 Galactosyltransferase in the absence of ligands
Method: X-RAY DIFFRACTION / Resolution: 2.57 Å

PDB-2vfz:
CRYSTAL STRUCTURE OF ALPHA-1,3 GALACTOSYLTRANSFERASE (R365K) IN COMPLEX WITH UDP-2F-GALACTOSE
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-UDP:
URIDINE-5'-DIPHOSPHATE / UDP*YM

ChemComp-MN:
Unknown entry

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-UPF:
URIDINE-5'-DIPHOSPHATE-2-DEOXY-2-FLUOROGALACTOSE

Source
  • bos taurus (domestic cattle)
KeywordsTRANSFERASE / SUBSTRATE SPECIFICITY / 3 GALACTOSYLTRANSFERASE / ALPHA-1 / ENZYME MECHANISM / GLYCOSYLTRANSFERASE GALACTOSYLTRANSFERASE / TRANSMEMBRANE / GOLGI APPARATUS / GLYCOPROTEIN / METAL-BINDING / SIGNAL-ANCHOR / MEMBRANE / MANGANESE / GLYCOSYLTRANSFERASE / GALACTOSYLTRANSFERASE / GT / R365K / ALPHA GT / GALACTOSE

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