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TitleUse of a 'Caged' Analog to Study Traffic of Choline within Acetylcholinesterase by Kinetic Crystallography
Journal, issue, pagesActa Crystallogr. ,Sect. D, Vol. 63, Page 1115-, Year 2007
Publish dateAug 22, 2007 (structure data deposition date)
AuthorsColletier, J.-P. / Royant, A. / Specht, A. / Sanson, B. / Nachon, F. / Masson, P. / Zaccai, G. / Sussman, J.L. / Goeldner, M. / Silman, I. ...Colletier, J.-P. / Royant, A. / Specht, A. / Sanson, B. / Nachon, F. / Masson, P. / Zaccai, G. / Sussman, J.L. / Goeldner, M. / Silman, I. / Bourgeois, D. / Weik, M.
External linksActa Crystallogr. ,Sect. D / PubMed:18007027
MethodsX-ray diffraction
Resolution2.4 - 3 Å
Structure data

PDB-2v96:
Structure of the unphotolysed complex of TcAChE with 1-(2- nitrophenyl)-2,2,2-trifluoroethyl-arsenocholine at 100K
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-2v97:
Structure of the unphotolysed complex of TcAChE with 1-(2- nitrophenyl)-2,2,2-trifluoroethyl-arsenocholine after a 9 seconds annealing to room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-2v98:
Structure of the complex of TcAChE with 1-(2-nitrophenyl)-2,2,2- trifluoroethyl-arsenocholine after a 9 seconds annealing to room temperature, during the first 5 seconds of which laser irradiation at 266nm took place
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-2va9:
Structure of native TcAChE after a 9 seconds annealing to room temperature during the first 5 seconds of which laser irradiation at 266nm took place
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-CFQ:
1-(2-nitrophenyl)-2,2,2-trifluoroethyl]-arsenocholine

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

Source
  • torpedo californica (Pacific electric ray)
KeywordsHYDROLASE / SERINE ESTERASE / ALTERNATIVE SPLICING / ACETYLCHOLINESTERASE CAGED COMPOUNDS KINETIC CRYSTALLOGRAPHY PARTIAL Q-WEIGHTED DIFFERENCE REFINEMENT BACKDOOR / NEUROTRANSMITTER DEGRADATION / LIPOPROTEIN / GLYCOPROTEIN / CELL JUNCTION / SYNAPSE / MEMBRANE / GPI-ANCHOR / PARTIAL Q-WEIGHTED DIFFERENCE REFINEMENT / KINETIC CRYSTALLOGRAPHY / CAGED COMPOUNDS / ACETYLCHOLINESTERASE / BACKDOOR

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