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TitleScaffolding as an organizing principle in trans-translation. The roles of small protein B and ribosomal protein S1.
Journal, issue, pagesJ Biol Chem, Vol. 282, Issue 9, Page 6356-6363, Year 2007
Publish dateMar 2, 2007
AuthorsReynald Gillet / Sukhjit Kaur / Wen Li / Marc Hallier / Brice Felden / Joachim Frank /
PubMed AbstractA eubacterial ribosome stalled on a defective mRNA can be released through a quality control mechanism referred to as trans-translation, which depends on the coordinating binding actions of transfer- ...A eubacterial ribosome stalled on a defective mRNA can be released through a quality control mechanism referred to as trans-translation, which depends on the coordinating binding actions of transfer-messenger RNA, small protein B, and ribosome protein S1. By means of cryo-electron microscopy, we obtained a map of the complex composed of a stalled ribosome and small protein B, which appears near the decoding center. This result suggests that, when lacking a codon, the A-site on the small subunit is a target for small protein B. To investigate the role of S1 played in trans-translation, we obtained a cryo-electron microscopic map, including a stalled ribosome, transfer-messenger RNA, and small protein Bs but in the absence of S1. In this complex, several connections between the 30 S subunit and transfer-messenger RNA that appear in the +S1 complex are no longer found. We propose the unifying concept of scaffolding for the roles of small protein B and S1 in binding of transfer-messenger RNA to the ribosome during trans-translation, and we infer a pathway of sequential binding events in the initial phase of trans-translation.
External linksJ Biol Chem / PubMed:17179154 / PubMed Central
MethodsEM (single particle)
Resolution11.8 - 13.6 Å
Structure data

1310

2ob7

EMDB-1310: Scaffolding as an organizing principle in trans-translation. The roles of small protein B and ribosomal protein S1.
PDB-2ob7: Structure of tmRNA-(SmpB)2 complex as inferred from cryo-EM
Method: EM (single particle) / Resolution: 11.8 Å

EMDB-1312:
Scaffolding as an organizing principle in trans-translation. The roles of small protein B and ribosomal protein S1.
Method: EM (single particle) / Resolution: 13.1 Å

Source
  • thermus thermophilus (bacteria)
KeywordsRNA BINDING PROTEIN/RNA / tmRNA / SmpB / RNA BINDING PROTEIN-RNA COMPLEX

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