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TitleAtomic structures of two novel immunoglobulin-like domain pairs in the actin cross-linking protein filamin.
Journal, issue, pagesJ Biol Chem, Vol. 284, Issue 37, Page 25450-25458, Year 2009
Publish dateSep 11, 2009
AuthorsOuti K Heikkinen / Salla Ruskamo / Peter V Konarev / Dmitri I Svergun / Tatu Iivanainen / Sami M Heikkinen / Perttu Permi / Harri Koskela / Ilkka Kilpeläinen / Jari Ylänne /
PubMed AbstractFilamins are actin filament cross-linking proteins composed of an N-terminal actin-binding domain and 24 immunoglobulin-like domains (IgFLNs). Filamins interact with numerous proteins, including the ...Filamins are actin filament cross-linking proteins composed of an N-terminal actin-binding domain and 24 immunoglobulin-like domains (IgFLNs). Filamins interact with numerous proteins, including the cytoplasmic domains of plasma membrane signaling and cell adhesion receptors. Thereby filamins mechanically and functionally link the cell membrane to the cytoskeleton. Most of the interactions have been mapped to the C-terminal IgFLNs 16-24. Similarly, as with the previously known compact domain pair of IgFLNa20-21, the two-domain fragments IgFLNa16-17 and IgFLNa18-19 were more compact in small angle x-ray scattering analysis than would be expected for two independent domains. Solution state NMR structures revealed that the domain packing in IgFLNa18-19 resembles the structure of IgFLNa20-21. In both domain pairs the integrin-binding site is masked, although the details of the domain-domain interaction are partly distinct. The structure of IgFLNa16-17 revealed a new domain packing mode where the adhesion receptor binding site of domain 17 is not masked. Sequence comparison suggests that similar packing of three tandem filamin domain pairs is present throughout the animal kingdom, and we propose that this packing is involved in the regulation of filamin interactions through a mechanosensor mechanism.
External linksJ Biol Chem / PubMed:19622754 / PubMed Central
MethodsSAS (X-ray synchrotron) / NMR (solution)
Structure data

SASDAS3:
Fila16-17 (immunoglobulin- like filamin two-domain fragment 16-17)
Method: SAXS/SANS

SASDAT3:
Fila18-19 (immunoglobulin- like filamin two-domain fragment 18-19)
Method: SAXS/SANS

SASDAU3:
Fila22-23 (immunoglobulin- like filamin two-domain fragment 22-23)
Method: SAXS/SANS

PDB-2k7p:
Filamin A Ig-like domains 16-17
Method: SOLUTION NMR

PDB-2k7q:
Filamin A Ig-like domains 18-19
Method: SOLUTION NMR

Source
  • Escherichia coli (E. coli)
  • homo sapiens (human)
KeywordsSTRUCTURAL PROTEIN / filamin / Ig-like / ABP-280 / actin binding protein / Acetylation / Actin-binding / Cytoplasm / Cytoskeleton / Disease mutation / Phosphoprotein / Polymorphism

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