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TitleExpanding the Thioglycoligase Strategy to the Synthesis of alpha-linked Thioglycosides Allows Structural Investigation of the Parent Enzyme/Substrate Complex
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 128, Page 2202-2203, Year 2006
Publish dateNov 16, 2005 (structure data deposition date)
AuthorsKim, Y.-W. / Lovering, A.L. / Chen, H. / Kantner, T. / McIntosh, L.P. / Strynadka, N.C.J. / Withers, S.G.
External linksJ. Am. Chem. Soc. / PubMed:16478160
MethodsX-ray diffraction
Resolution1.95 Å
Structure data

PDB-2f2h:
Structure of the YicI thiosugar Michaelis complex
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-XTG:
4-NITROPHENYL 6-THIO-6-S-ALPHA-D-XYLOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE

ChemComp-GOL:
GLYCEROL

ChemComp-MPO:
3[N-MORPHOLINO]PROPANE SULFONIC ACID / pH buffer*YM

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
KeywordsHYDROLASE / beta8alpha8 barrel

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