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Structure paper

TitleStructures of the G81A mutant form of the active chimera of (S)-mandelate dehydrogenase and its complex with two of its substrates
Journal, issue, pagesActa Crystallogr. ,Sect. D, Vol. 65, Page 543-552, Year 2009
Publish dateJul 5, 2005 (structure data deposition date)
AuthorsSukumar, N. / Dewanti, A. / Merli, A. / Rossi, G.L. / Mitra, B. / Mathews, F.S.
External linksActa Crystallogr. ,Sect. D / PubMed:19465768
MethodsX-ray diffraction
Resolution1.6 - 2.5 Å
Structure data

PDB-2a7n:
Crystal Structure of the G81A mutant of the Active Chimera of (S)-Mandelate Dehydrogenase
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-2a7p:
Crystal Structure of the G81A mutant of the Active Chimera of (S)-Mandelate Dehydrogenase in complex with its substrate 3-indolelactate
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-2a85:
Crystal Structure of the G81A mutant of the Active Chimera of (S)-Mandelate Dehydrogenase in complex with its substrate 2-hydroxyoctanoate
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-3giy:
Crystal Structures of the G81A Mutant of the Active Chimera of (S)-Mandelate Dehydrogenase and its Complex with Two of its Substrates
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-HOH:
WATER

ChemComp-3IL:
3-(INDOL-3-YL) LACTATE

ChemComp-HOC:
(2S)-2-HYDROXYOCTANOIC ACID

Source
  • pseudomonas putida (bacteria)
  • spinacia oleracea (spinach)
KeywordsOXIDOREDUCTASE / TIM BARREL / Hydroxy acid oxidizing enzyme

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