Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM structures of CCHFV polymerase reveal a stepwise initiation stabilization pathway and a dual-site inhibition mechanism.
Journal, issue, pages	Cell Rep, Vol. 45, Issue 9, Page 117945, Year 2026
Publish date	Sep 3, 2026
Authors	Kankan Yang / Haiqiang Wu / Xunbi Liu / Zuxing Liang / Junwei Zou / Yong Wang / Jun Ma /
PubMed Abstract	Crimean-Congo hemorrhagic fever virus (CCHFV) is a high-priority pathogen with high case-fatality rates, yet approved therapeutics remain unavailable. The CCHFV L segment encodes a ∼450-kDa RNA- ...Crimean-Congo hemorrhagic fever virus (CCHFV) is a high-priority pathogen with high case-fatality rates, yet approved therapeutics remain unavailable. The CCHFV L segment encodes a ∼450-kDa RNA-dependent RNA polymerase (L protein) orchestrating viral replication, but its structural mechanisms remain elusive. Here, we present high-resolution cryo-EM structures of the CCHFV L protein in apo, 5' vRNA-bound, 5'/3' promoter-bound, and inhibitor-bound states. The catalytic core exhibits the canonical architecture of the Bunyavirales order, featuring conserved motifs and coordinated promoter recognition via a 5' vRNA "hook" and a secondary 3' vRNA-binding site. Using suramin as a probe, we identified a dual-site mechanism of polymerase inhibition. Suramin competitively occludes the 5' vRNA-binding pocket through electrostatic mimicry of the RNA backbone and concurrently traps a distal linker-fingers interface, restricting the conformational dynamics required for catalysis. Collectively, these findings provide structural insights into CCHFV polymerase regulation and inhibition.
External links	Cell Rep / PubMed:42696455
Methods	EM (single particle)
Resolution	2.5 - 3.03 Å
Structure data	68655 22te EMDB-68655, PDB-22te: Cryo-EM structure of Crimean-Congo hemorrhagic fever virus RNA polymerase in apo state Method: EM (single particle) / Resolution: 2.59 Å 68657 22th EMDB-68657, PDB-22th: Cryo-EM structure of Crimean-Congo hemorrhagic fever virus RNA polymerase in complex with suramin Method: EM (single particle) / Resolution: 2.5 Å 68663 22tr EMDB-68663, PDB-22tr: Cryo-EM structure of Crimean-Congo hemorrhagic fever virus RNA polymerase in complex with 5' vRNA promotor Method: EM (single particle) / Resolution: 2.75 Å 80706 26jz EMDB-80706, PDB-26jz: Cryo-EM structure of Crimean-Congo hemorrhagic fever virus RNA polymerase in complex with 5'/3' vRNA dual-promotor Method: EM (single particle) / Resolution: 2.65 Å 80707 26ka EMDB-80707, PDB-26ka: Cryo-EM structure of Crimean-Congo hemorrhagic fever virus RNA polymerase in complex with the 5' vRNA promotor from the 5'/3' vRNA dual-promoter dataset Method: EM (single particle) / Resolution: 3.03 Å 80708 26kb EMDB-80708, PDB-26kb: Cryo-EM structure of Crimean-Congo hemorrhagic fever virus RNA polymerase in the apo3 state from the 5'/3' vRNA dual-promoter dataset Method: EM (single particle) / Resolution: 2.76 Å 80710 26kd EMDB-80710, PDB-26kd: Cryo-EM structure of Crimean-Congo hemorrhagic fever virus RNA polymerase in the apo2 state from the 5'/3' vRNA dual-promoter dataset Method: EM (single particle) / Resolution: 2.64 Å
Chemicals	ChemComp-ZN: Unknown entry ChemComp-MN: Unknown entry ChemComp-MG: Unknown entry ChemComp-SVR: 8,8'-[CARBONYLBIS[IMINO-3,1-PHENYLENECARBONYLIMINO(4-METHYL-3,1-PHENYLENE)CARBONYLIMINO]]BIS-1,3,5-NAPHTHALENETRISULFON / medication*YM
Source	orthonairovirus haemorrhagiae crimean-congo hemorrhagic fever virus strain ibar10200 Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970)
Keywords	RNA BINDING PROTEIN / Polymerase / RNA BINDING PROTEIN/RNA / RNA BINDING PROTEIN-RNA complex