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TitleCryoSeek identification of glycofibrils with diverse compositions and structural assemblies.
Journal, issue, pagesCell Chem Biol, Vol. 33, Issue 5, Page 699-710.e2, Year 2026
Publish dateMay 21, 2026
AuthorsZhangqiang Li / Tongtong Wang / Yitong Sun / Kui Xu / Wenze Huang / Qiangfeng Cliff Zhang / Chuangye Yan / Mingxu Hu / Nieng Yan /
PubMed AbstractCryoSeek is a research strategy that employs cryo-electron microscopy (cryo-EM) to discover bio-entities from accessible sources, supplemented with AI-facilitated data processing and bioinformatic ...CryoSeek is a research strategy that employs cryo-electron microscopy (cryo-EM) to discover bio-entities from accessible sources, supplemented with AI-facilitated data processing and bioinformatic analyses. Here we report CryoSeek-based characterization of more glycofibrils isolated from the Tsinghua Lotus Pond (TLP), named TLP-0/2/3/4b/IPT, with resolutions ranging from 3.0-3.5 Å. These glycofibrils, all covered with dense glycoshells, have various mass percentiles of the central protein components. TLP-0 has no protein at all, TLP-2 and TLP-4b each only have a linear chain of di- or tetra-peptide repeats, respectively; the central stem of TLP-3 is a trimer of linear tripeptide repeats, and IPT (immunoglobulin-like, plexins, and transcription factors) refers to the tandem domain that constitutes the central stem of TLP-IPT. Glycan-mediated interactions determine the structural assembly of the glycofibrils that lack folded protein component. Our previous and current studies reveal the diversity of the structural folds of glycans, advance our understanding of glycan architecture, and further demonstrate CryoSeek as a structure-first paradigm for discovery.
External linksCell Chem Biol / PubMed:42013841
MethodsEM (helical sym.)
Resolution3.1 - 3.5 Å
Structure data

67848

21nr

EMDB-67848, PDB-21nr:
Cryo-EM structure of TLP-IPT
Method: EM (helical sym.) / Resolution: 3.3 Å

67849

21ns

EMDB-67849, PDB-21ns:
Cryo-EM structure of TLP-4b
Method: EM (helical sym.) / Resolution: 3.3 Å

67850

21nt

EMDB-67850, PDB-21nt:
Cryo-EM structure of TLP-3
Method: EM (helical sym.) / Resolution: 3.5 Å

68122

21zt

EMDB-68122, PDB-21zt:
Cryo-EM structure of TLP-2
Method: EM (helical sym.) / Resolution: 3.5 Å

68132

22ag

EMDB-68132, PDB-22ag:
Cryo-EM structure of TLP-0
Method: EM (helical sym.) / Resolution: 3.1 Å

Source
  • algae metagenome (others)
KeywordsCARBOHYDRATE / Glycofibril

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