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TitleCrystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 101, Page 16186-16191, Year 2004
Publish dateAug 23, 2004 (structure data deposition date)
AuthorsDutta, S. / Li, Y. / Johnson, D. / Dzantiev, L. / Richardson, C.C. / Romano, L.J. / Ellenberger, T.
External linksProc. Natl. Acad. Sci. USA / PubMed:15528277
MethodsX-ray diffraction
Resolution2.1 - 2.7 Å
Structure data

PDB-1x9m:
T7 DNA polymerase in complex with an N-2-acetylaminofluorene-adducted DNA
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-1x9s:
T7 DNA polymerase in complex with a primer/template DNA containing a disordered N-2 aminofluorene on the template, crystallized with dideoxy-CTP as the incoming nucleotide.
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-1x9w:
T7 DNA polymerase in complex with a primer/template DNA containing a disordered N-2 aminofluorene on the template, crystallized with dideoxy-ATP as the incoming nucleotide.
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • enterobacteria phage t7 (virus)
  • escherichia coli (E. coli)
KeywordsTRANSFERASE/ELECTRON TRANSPORT/DNA / DNA ploymerase / N-2-acetylaminofluorene / Replication block / Mutagenesis / TRANSFERASE-ELECTRON TRANSPORT-DNA COMPLEX / DNA polymerase / N-2-aminofluorene

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