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Structure paper

TitleActivation process of [NiFe] hydrogenase elucidated by high-resolution X-Ray analyses: conversion of the ready to the unready state
Journal, issue, pagesStructure, Vol. 13, Page 1635-1642, Year 2005
Publish dateDec 7, 2004 (structure data deposition date)
AuthorsOgata, H. / Hirota, S. / Nakahara, A. / Komori, H. / Shibata, N. / Kato, T. / Kano, K. / Higuchi, Y.
External linksStructure / PubMed:16271886
MethodsX-ray diffraction
Resolution1.04 - 1.5 Å
Structure data

PDB-1wui:
Ultra-High resolution Structure Of The Ni-A State Of [Nife]Hydrogenase From Desulufovibrio Vulgaris Miyazaki F
Method: X-RAY DIFFRACTION / Resolution: 1.04 Å

PDB-1wuj:
Three-Dimensional Structure Of The Ni-B State Of [Nife]Hydrogenase From Desulufovibrio Vulgaris Miyazaki F
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-1wuk:
High resolution Structure Of The Oxidized State Of [Nife]Hydrogenase From Desulufovibrio Vulgaris Miyazaki F
Method: X-RAY DIFFRACTION / Resolution: 1.1 Å

PDB-1wul:
High Resolution Structure Of The Reduced State Of [Nife]Hydrogenase From Desulufovibrio Vulgaris Miyazaki F
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-F3S:
FE3-S4 CLUSTER

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-MG:
Unknown entry

ChemComp-NFC:
NI-FE ACTIVE CENTER A-FORM

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-NFO:
NI-FE OXIDIZED ACTIVE CENTER

ChemComp-NFR:
NI-FE REDUCED ACTIVE CENTER

Source
  • desulfovibrio vulgaris str. 'miyazaki f' (bacteria)
KeywordsOXIDOREDUCTASE / High resolution crystal structure / [NiFe]hydrogenase / Ni-A state / Unready state / Ni-B state / Ready state / oxidized state / reduced state

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