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Structure paper

TitleUnexpected Oxidation of a Depsipeptide Substrate Analogue in Crystalline Isopenicillin N Synthase.
Journal, issue, pagesChembiochem, Vol. 7, Page 351-, Year 2006
Publish dateJul 20, 2004 (structure data deposition date)
AuthorsDaruzzaman, A. / Clifton, I.J. / Adlington, R.M. / Baldwin, J.E. / Rutledge, P.J.
External linksChembiochem / PubMed:16444759
MethodsX-ray diffraction
Resolution1.4 - 1.46 Å
Structure data

PDB-1w3v:
Isopenicillin N synthase d-(L-a-aminoadipoyl)-(3R)-methyl-L-cysteine D-a-hydroxyisovaleryl ester complex (anaerobic)
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-1w3x:
Isopenicillin N synthase d-(L-a-aminoadipoyl)-(3R)-methyl-L-cysteine D-a-hydroxyisovaleryl ester complex (Oxygen exposed 5 minutes 20 bar)
Method: X-RAY DIFFRACTION / Resolution: 1.46 Å

Chemicals

ChemComp-FE2:
Unknown entry

ChemComp-MDZ:
N~6~-METHYL-6-OXO-L-LYSINE - 2-[(3-MERCAPTOBUTANOYL)OXY]-3-METHYLBUTANOIC ACID

ChemComp-HOH:
WATER

ChemComp-W2X:
N~6~-[(1R)-1-({[(1R,2R)-1-CARBOXY-3-HYDROXY-2-METHYLPROPYL]OXY}CARBONYL)-2-MERCAPTOPROP-2-EN-1-YL]-6-OXO-L-LYSINE

Source
  • emericella nidulans (strain fgsc a4 / atcc 38163 / cbs 112.46 / nrrl 194 / m139) (mold)
KeywordsSYNTHASE / ANTIBIOTIC BIOSYNTHESIS / B-LACTAM ANTIBIOTIC / IRON / OXIDOREDUCTASE / OXYGENASE / PENICILLIN BIOSYNTHESIS

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