Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM structures of Candida albicans chitin synthase Chs1 reveal a druggable translocation channel.
Journal, issue, pages	Nat Microbiol, Year 2026
Publish date	Sep 15, 2026
Authors	Zhenning Ren / Abhishek Chhetri / Chang Liu / Ziqiang Guan / ShuYu Offner / Dmitry Kozhanov / Mario J Borgnia / Wonpil Im / Kenichi Yokoyama / Seok-Yong Lee /
PubMed Abstract	Invasive candidiasis is a leading cause of hospital-acquired bloodstream infections with high mortality. While the fungal cell wall is an excellent therapeutic target, inhibitor development against ...Invasive candidiasis is a leading cause of hospital-acquired bloodstream infections with high mortality. While the fungal cell wall is an excellent therapeutic target, inhibitor development against the essential chitin synthase (Chs) has been hampered by the absence of structural and mechanistic understanding of class II Chs, which contribute to fungal viability. Here we present cryo-electron microscopy structures of Candida albicans class II Chs (CaChs1) at 2.93-3.38 Å resolution, providing insights into its mechanisms of early elongation, chito-oligomer translocation and inhibition by the CaChs1-specific non-competitive inhibitor diynyl arylamine (DA). Chitin elongation and translocation are coupled to coordinated motion of the glycosyltransferase domain and the dimer interface. Notably, DA binds within the chitin translocation channel where a regulatory lipid resides and inhibits the enzyme by occluding product polymer extrusion. Importantly, DA showed potent synergy with the class I Chs inhibitor nikkomycin Z against C. albicans and Candida auris. These findings establish the chitin translocation channel as a druggable site for rational antifungal design.
External links	Nat Microbiol / PubMed:42744982 / PubMed Central
Methods	EM (single particle)
Resolution	2.85 - 3.38 Å
Structure data	76826 12xf EMDB-76826, PDB-12xf: Structure of Class II chitin synthase, Chs1, from Candida albicans (CaChs1) in the apo state Method: EM (single particle) / Resolution: 3.02 Å 76827 12xg EMDB-76827, PDB-12xg: Structure of Class II chitin synthase, Chs1, from Candida albicans (CaChs1), state 1 (post UDP hydrolysis) after 5 min reaction Method: EM (single particle) / Resolution: 3.38 Å 76828 12xh EMDB-76828, PDB-12xh: Structure of Class II chitin synthase, Chs1, from Candida albicans (CaChs1), state 2 (pre-translocation) after 5 min reaction Method: EM (single particle) / Resolution: 2.93 Å 76829 12xi EMDB-76829, PDB-12xi: Structure of Class II chitin synthase, Chs1, from Candida albicans (CaChs1), state 3 (post-translocation) after 5 min reaction Method: EM (single particle) / Resolution: 3.24 Å 76830 12xj EMDB-76830, PDB-12xj: Structure of Class II chitin synthase, Chs1, from Candida albicans (CaChs1), bound to diynyl arylamine (DA, aka RO-09-3143) Method: EM (single particle) / Resolution: 2.93 Å 76831 12xk EMDB-76831, PDB-12xk: Candida albicans Chitin synthase 1 (CaChs1) bound to UDP-GlcNAc and diynyl arylamine (DA, aka RO-09-3143) Method: EM (single particle) / Resolution: 2.96 Å 76832 12xl EMDB-76832, PDB-12xl: Candida albicans Chitin synthase 1 (CaChs1) intermediate state (DA-2), bound to UDP and diynyl arylamine (DA, aka RO-09-3143) Method: EM (single particle) / Resolution: 3.02 Å 76833 12xm EMDB-76833, PDB-12xm: Candida albicans Chitin synthase 1 (CaChs1) intermediate state (DA-3), bound to UDP and diynyl arylamine (DA, aka RO-09-3143) Method: EM (single particle) / Resolution: 3.0 Å 76834 12xn EMDB-76834, PDB-12xn: Candida albicans Chitin synthase 1 (CaChs1) intermediate state (DA-4), bound to UDP and diynyl arylamine (DA, aka RO-09-3143) Method: EM (single particle) / Resolution: 3.1 Å 76835 12xo EMDB-76835, PDB-12xo: Candida albicans Chitin synthase 1 (CaChs1) intermediate state (DA-5), bound to UDP and diynyl arylamine (DA, aka RO-09-3143) Method: EM (single particle) / Resolution: 2.85 Å 76836 12xp EMDB-76836, PDB-12xp: Candida albicans Chitin synthase 1 (CaChs1) intermediate state (DA-6), bound to UDP and diynyl arylamine (DA, aka RO-09-3143) Method: EM (single particle) / Resolution: 3.29 Å 76837 12xq EMDB-76837, PDB-12xq: Candida albicans Chitin synthase 1 (CaChs1) intermediate state (DA-7), bound to UDP and diynyl arylamine (DA, aka RO-09-3143) Method: EM (single particle) / Resolution: 3.1 Å
Chemicals	ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipidYM ChemComp-MG: Unknown entry ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose ChemComp-UDP: URIDINE-5'-DIPHOSPHATE / UDPYM ChemComp-HOH: WATER PDB-1deh: CRYSTALLIZATION OF HUMAN BETA1 ALCOHOL DEHYDROGENASE (15 MG/ML) IN 50 MM SODIUM PHOSPHATE (PH 7.5), 2.0 MM NAD+ AND 1 MM 4-IODOPYRAZOLE AT 25 OC, 13% (W/V) PEG 8000 ChemComp-UD1: URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE
Source	candida albicans (yeast)
Keywords	MEMBRANE PROTEIN / Enzyme / chitin synthesis