EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Functional and structural basis of Omicron BA.3.2.1 spike.
ジャーナル・号・ページ	Cell Rep, Vol. 45, Issue 8, Page 117812, Year 2026
掲載日	2026年8月8日
著者	Yan Wang / Yanping Hu / Zhenhang Chen / Jing Zou / Ke Zhang / Ping Ren / Pei-Yong Shi / Bo Liang / Xuping Xie /
PubMed 要旨	SARS-CoV-2 BA.3.2 sublineages, derived from BA.3 and carrying substantial spike divergence, raised concerns about altered fitness and antigenicity. Using BA.3.2.1 as a representative strain, we ...SARS-CoV-2 BA.3.2 sublineages, derived from BA.3 and carrying substantial spike divergence, raised concerns about altered fitness and antigenicity. Using BA.3.2.1 as a representative strain, we engineered live-attenuated SARS-CoV-2 encoding BA.3.2.1, LP.8.1, or XEC spikes and benchmarked them against BA.3 and KP.3. BA.3.2.1 outcompetes BA.3 in primary human airway epithelium but replicates less efficiently than JN.1 descendants and shows the greatest resistance to neutralization by KP.2/KP.3 convalescent sera. Although BA.3.2.1 RBD binds hACE2 with high affinity, its trimeric spike engages hACE2 less efficiently than LP.8.1. Cryoelectron microscopy structures reveal that BA.3.2.1 spike predominantly adopts a compact, asymmetric closed conformation stabilized by protomer rearrangements, N-linked glycosylation, and a distinct fusion-peptide-proximal region. This architecture increases spike stability, limits receptor engagement, reduces fusogenicity, and masks antibody-sensitive epitopes. Thus, BA.3.2.1 enhances immune evasion at the cost of replication fitness, providing a structural-functional explanation for BA.3.2's limited prevalence and underscoring the need for continued variant surveillance.
リンク	Cell Rep / PubMed:42571698
手法	EM (単粒子)
解像度	2.35 - 2.82 Å
構造データ	76493 12jt EMDB-76493, PDB-12jt: SARS-CoV-2 BA.3.2.1 Spike with N529Q mutation 手法: EM (単粒子) / 解像度: 2.4 Å 76501 12jz EMDB-76501, PDB-12jz: Cryo-EM structure of SARS-CoV-2 BA.3.2.1 Spike with K852A mutation, closed conformation 手法: EM (単粒子) / 解像度: 2.35 Å EMDB-76694: Cryo-EM structure of SARS-CoV-2 BA.3.2.1 spike with K852A mutation, flexible conformation 手法: EM (単粒子) / 解像度: 2.73 Å EMDB-76706: Cryo-EM structure of SARS-CoV-2 BA.3.2.1 spike with N529Q mutation, flexible conformation 手法: EM (単粒子) / 解像度: 2.82 Å 76713 12rp EMDB-76713, PDB-12rp: Local refinement of RBDA, RBDC, and NTDB of SARS-CoV-2 BA.3.2.1 spike with K852A mutation, closed conformation 手法: EM (単粒子) / 解像度: 2.78 Å EMDB-76849: Cryo-EM structure of SARS-CoV-2 BA.3.2.1 spike with K852A mutation, open conformation 手法: EM (単粒子) / 解像度: 2.73 Å EMDB-76850: Cryo-EM structure of SARS-CoV-2 BA.3.2.1 spike with N529Q mutation, open conformation 手法: EM (単粒子) / 解像度: 2.73 Å 76936 13bd EMDB-76936, PDB-13bd: Local refinement of the RBD and NTD in the closed BA.3.2.1 spike with N529Q mutant 手法: EM (単粒子) / 解像度: 2.65 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose
由来	severe acute respiratory syndrome coronavirus 2 (ウイルス)
キーワード	VIRAL PROTEIN / SARS-COV-2