Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	The bacterial mechanosensitive channel MscM gates through concerted changes in its transmembrane and cytoplasmic domains.
Journal, issue, pages	Nat Commun, Year 2026
Publish date	Jul 22, 2026
Authors	Giorgos Hiotis / Thomas Walz /
PubMed Abstract	The mechanosensitive channel of small conductance (MscS) is the founding member of the family of MscS-like channels, which share a structurally conserved core but feature additional structural ...The mechanosensitive channel of small conductance (MscS) is the founding member of the family of MscS-like channels, which share a structurally conserved core but feature additional structural elements that define their specific channel characteristics. Here, we characterize the structure and function of the Escherichia coli mechanosensitive channel of mini conductance (MscM), which features eight additional transmembrane (TM) helices and a large periplasmic domain. Our cryo-EM structures reveal that channel gating involves conformational changes in all domains of MscM. In particular, a cytoplasmic extension of TM7 couples the conformation of the TM domain to that of the cytoplasmic domain, resulting in gating of its lateral fenestrations, where ions enter the channel. Thus, different from all other MscS-like channels studied to date, channel gating in MscM is mediated by its cytoplasmic domain and not the TM domain, which senses changes in membrane tension and operates the cytoplasmic gates.
External links	Nat Commun / PubMed:42481478
Methods	EM (single particle)
Resolution	2.8 - 6.7 Å
Structure data	EMDB-76005: EcMscM in NaCl in a closed conformation Method: EM (single particle) / Resolution: 6.7 Å 76007 11sm EMDB-76007, PDB-11sm: Core of EcMscM in NaCl in a closed conformation Method: EM (single particle) / Resolution: 3.4 Å 76009 11so EMDB-76009, PDB-11so: EcMscM lacking the first periplasmic helical bundle in NaCl in a closed conformation Method: EM (single particle) / Resolution: 4.3 Å EMDB-76010: Core of EcMscM lacking the first periplasmic helical bundle in NaCl in a closed conformation Method: EM (single particle) / Resolution: 3.4 Å EMDB-76011: EcMscM in KCl in an open conformation Method: EM (single particle) / Resolution: 2.8 Å EMDB-76013: EcMscM in KCl in an open conformation, C1 processed Method: EM (single particle) / Resolution: 3.2 Å EMDB-76015: EcMscM in KCl in an open conformation with density for the second periplasmic helical bundle Method: EM (single particle) / Resolution: 3.3 Å 76016 11sq EMDB-76016, PDB-11sq: EcMscM in KCl in an open conformation, composite structure Method: EM (single particle) / Resolution: 3.2 Å EMDB-76017: EcMscM lacking the first periplasmic helical bundle in KCl in an open conformation Method: EM (single particle) / Resolution: 3.2 Å 76018 11sr EMDB-76018, PDB-11sr: EcMscM lacking the first periplasmic helical bundle and the cytoplasmic extension of TM7 in NaCl in a closed conformation Method: EM (single particle) / Resolution: 5.1 Å EMDB-76019: Core of EcMscM lacking the first periplasmic helical bundle and the cytoplasmic extension of TM7 in NaCl in a closed conformation Method: EM (single particle) / Resolution: 3.5 Å
Source	escherichia coli k-12 (bacteria)
Keywords	MEMBRANE PROTEIN / Mechanosensitive / Ion channel / MscS