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Structure paper

TitleStructural mechanism of the active bicarbonate transporter from cyanobacteria.
Journal, issue, pagesNat Plants, Vol. 5, Issue 11, Page 1184-1193, Year 2019
Publish dateNov 11, 2019
AuthorsChengcheng Wang / Bo Sun / Xue Zhang / Xiaowei Huang / Minhua Zhang / Hui Guo / Xin Chen / Fang Huang / Taiyu Chen / Hualing Mi / Fang Yu / Lu-Ning Liu / Peng Zhang /
PubMed AbstractBicarbonate transporters play essential roles in pH homeostasis in mammals and photosynthesis in aquatic photoautotrophs. A number of bicarbonate transporters have been characterized, among which is ...Bicarbonate transporters play essential roles in pH homeostasis in mammals and photosynthesis in aquatic photoautotrophs. A number of bicarbonate transporters have been characterized, among which is BicA-a low-affinity, high-flux SLC26-family bicarbonate transporter involved in cyanobacterial CO-concentrating mechanisms (CCMs) that accumulate CO and improve photosynthetic carbon fixation. Here, we report the three-dimensional structure of BicA from Synechocystis sp. PCC6803. Crystal structures of the transmembrane domain (BicA) and the cytoplasmic STAS domain (BicA) of BicA were solved. BicA was captured in an inward-facing HCO-bound conformation and adopts a '7+7' fold monomer. HCO binds to a cytoplasm-facing hydrophilic pocket within the membrane. BicA is assembled as a compact homodimer structure and is required for the dimerization of BicA. The dimeric structure of BicA was further analysed using cryo-electron microscopy and physiological analysis of the full-length BicA, and may represent the physiological unit of SLC26-family transporters. Comparing the BicA structure with the outward-facing transmembrane domain structures of other bicarbonate transporters suggests an elevator transport mechanism that is applicable to the SLC26/4 family of sodium-dependent bicarbonate transporters. This study advances our knowledge of the structures and functions of cyanobacterial bicarbonate transporters, and will inform strategies for bioengineering functional BicA in heterologous organisms to increase assimilation of CO.
External linksNat Plants / PubMed:31712753
MethodsEM (single particle) / X-ray diffraction
Resolution2.197 - 11.9 Å
Structure data

EMDB-9897:
Bicarbonate transporter BicA dimer
Method: EM (single particle) / Resolution: 11.9 Å

PDB-6ki1:
The transmembrane domain of a cyanobacterium bicarbonate transporter BicA
Method: X-RAY DIFFRACTION / Resolution: 2.809 Å

PDB-6ki2:
The STAS domain of cyanobacteria bicarbonate transporter BicA
Method: X-RAY DIFFRACTION / Resolution: 2.197 Å

Chemicals

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

ChemComp-NA:
Unknown entry

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

Source
  • synechocystis sp. pcc 6803 (bacteria)
KeywordsTRANSPORT PROTEIN / Bicarbonate transporter / CO2-concentrating mechanisms (CCM) / cyanobacteria / solute carrier (SLC) / STAS domain

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