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TitleCFAP70 Is a Novel Axoneme-Binding Protein That Localizes at the Base of the Outer Dynein Arm and Regulates Ciliary Motility.
Journal, issue, pagesCells, Vol. 7, Issue 9, Year 2018
Publish dateAug 29, 2018
AuthorsNoritoshi Shamoto / Keishi Narita / Tomohiro Kubo / Toshiyuki Oda / Sen Takeda /
PubMed AbstractIn the present study, we characterized CFAP70, a candidate of cilia-related protein in mice. As this protein has a cluster of tetratricopeptide repeat (TPR) domains like many components of the ...In the present study, we characterized CFAP70, a candidate of cilia-related protein in mice. As this protein has a cluster of tetratricopeptide repeat (TPR) domains like many components of the intraflagellar transport (IFT) complex, we investigated the domain functions of particular interest in ciliary targeting and/or localization. RT-PCR and immunohistochemistry of various mouse tissues demonstrated the association of CFAP70 with motile cilia and flagella. A stepwise extraction of proteins from swine tracheal cilia showed that CFAP70 bound tightly to the ciliary axoneme. Fluorescence microscopy of the cultured ependyma expressing fragments of CFAP70 demonstrated that the N-terminus rather than the C-terminus with the TPR domains was more important for the ciliary localization. When CFAP70 was knocked down in cultured mouse ependyma, reductions in cilia beating frequency were observed. Consistent with these observations, a mutant lacking the CFAP70 homolog, FAP70, showed defects in outer dynein arm (ODA) activity and a reduction in flagellar motility. Cryo-electron tomography revealed that the N-terminus of FAP70 resided stably at the base of the ODA. These results demonstrated that CFAP70 is a novel regulatory component of the ODA in motile cilia and flagella, and that the N-terminus is important for its ciliary localization.
External linksCells / PubMed:30158508 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution40.0 Å
Structure data

EMDB-9618:
Localization of FAP70 N-terminus on Chlamydomonas axoneme
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

Source
  • Chlamydomonas reinhardtii (plant)

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