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TitleCryo-EM structure of TRPC5 at 2.8-Å resolution reveals unique and conserved structural elements essential for channel function.
Journal, issue, pagesSci Adv, Vol. 5, Issue 7, Page eaaw7935, Year 2019
Publish dateJul 24, 2019
AuthorsJingjing Duan / Jian Li / Gui-Lan Chen / Yan Ge / Jieyu Liu / Kechen Xie / Xiaogang Peng / Wei Zhou / Jianing Zhong / Yixing Zhang / Jie Xu / Changhu Xue / Bo Liang / Lan Zhu / Wei Liu / Cheng Zhang / Xiao-Li Tian / Jianbin Wang / David E Clapham / Bo Zeng / Zongli Li / Jin Zhang /
PubMed AbstractThe transient receptor potential canonical subfamily member 5 (TRPC5), one of seven mammalian TRPC members, is a nonselective calcium-permeant cation channel. TRPC5 is of considerable interest as a ...The transient receptor potential canonical subfamily member 5 (TRPC5), one of seven mammalian TRPC members, is a nonselective calcium-permeant cation channel. TRPC5 is of considerable interest as a drug target in the treatment of progressive kidney disease, depression, and anxiety. Here, we present the 2.8-Å resolution cryo-electron microscopy (cryo-EM) structure of the mouse TRPC5 (mTRPC5) homotetramer. Comparison of the TRPC5 structure to previously determined structures of other TRPC and TRP channels reveals differences in the extracellular pore domain and in the length of the S3 helix. The disulfide bond at the extracellular side of the pore and a preceding small loop are essential elements for its proper function. This high-resolution structure of mTRPC5, combined with electrophysiology and mutagenesis, provides insight into the lipid modulation and gating mechanisms of the TRPC family of ion channels.
External linksSci Adv / PubMed:31355338 / PubMed Central
MethodsEM (single particle)
Resolution2.89 Å
Structure data

9615

6aei

EMDB-9615, PDB-6aei:
Cryo-EM structure of the receptor-activated TRPC5 ion channel
Method: EM (single particle) / Resolution: 2.89 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

ChemComp-LPP:
2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE / phospholipid*YM

Source
  • mus musculus (house mouse)
KeywordsMEMBRANE PROTEIN / Cryo-EM / mouse TRPC5 / ion channel

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