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TitleCryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchange.
Journal, issue, pagesNat Struct Mol Biol, Vol. 24, Issue 1, Page 40-46, Year 2017
Publish dateDec 12, 2016
AuthorsJingfei Xu / Lingyun Zhao / Yuanyuan Xu / Weixing Zhao / Patrick Sung / Hong-Wei Wang /
PubMed AbstractThe central step in eukaryotic homologous recombination (HR) is ATP-dependent DNA-strand exchange mediated by the Rad51 recombinase. In this process, Rad51 assembles on single-stranded DNA (ssDNA) ...The central step in eukaryotic homologous recombination (HR) is ATP-dependent DNA-strand exchange mediated by the Rad51 recombinase. In this process, Rad51 assembles on single-stranded DNA (ssDNA) and generates a helical filament that is able to search for and invade homologous double-stranded DNA (dsDNA), thus leading to strand separation and formation of new base pairs between the initiating ssDNA and the complementary strand within the duplex. Here, we used cryo-EM to solve the structures of human RAD51 in complex with DNA molecules, in presynaptic and postsynaptic states, at near-atomic resolution. Our structures reveal both conserved and distinct structural features of the human RAD51-DNA complexes compared with their prokaryotic counterpart. Notably, we also captured the structure of an arrested synaptic complex. Our results provide new insight into the molecular mechanisms of the DNA homology search and strand-exchange processes.
External linksNat Struct Mol Biol / PubMed:27941862 / PubMed Central
MethodsEM (helical sym.)
Resolution4.4 - 14.0 Å
Structure data

9566

5h1b

EMDB-9566, PDB-5h1b:
Human RAD51 presynaptic complex
Method: EM (helical sym.) / Resolution: 4.4 Å

9567

5h1c

EMDB-9567, PDB-5h1c:
Human RAD51 post-synaptic complexes
Method: EM (helical sym.) / Resolution: 4.5 Å

EMDB-9568:
human RAD51 presynaptic complex
Method: EM (helical sym.) / Resolution: 14.0 Å

Chemicals

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

Source
  • human (human)
  • homo sapiens (human)
KeywordsDNA BINDING PROTEIN/DNA / DNA repair / ATPase / homologous recombination / DNA BINDING PROTEIN-DNA complex

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