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TitleElectron cryo-tomography provides insight into procentriole architecture and assembly mechanism.
Journal, issue, pagesElife, Vol. 8, Year 2019
Publish dateFeb 11, 2019
AuthorsSam Li / Jose-Jesus Fernandez / Wallace F Marshall / David A Agard /
PubMed AbstractCentriole is an essential structure with multiple functions in cellular processes. Centriole biogenesis and homeostasis is tightly regulated. Using electron cryo-tomography (cryoET) we present the ...Centriole is an essential structure with multiple functions in cellular processes. Centriole biogenesis and homeostasis is tightly regulated. Using electron cryo-tomography (cryoET) we present the structure of procentrioles from . We identified a set of non-tubulin components attached to the triplet microtubule (MT), many are at the junctions of tubules likely to reinforce the triplet. We describe structure of the A-C linker that bridges neighboring triplets. The structure infers that POC1 is likely an integral component of A-C linker. Its conserved WD40 β-propeller domain provides attachment sites for other A-C linker components. The twist of A-C linker results in an iris diaphragm-like motion of the triplets in the longitudinal direction of procentriole. Finally, we identified two assembly intermediates at the growing ends of procentriole allowing us to propose a model for the procentriole assembly. Our results provide a comprehensive structural framework for understanding the molecular mechanisms underpinning procentriole biogenesis and assembly.
External linksElife / PubMed:30741631 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution21.4 - 23.1 Å
Structure data

EMDB-9167:
Electron cryo-tomography and subtomogram averaging of microtubule triplet from procentriole
Method: EM (subtomogram averaging) / Resolution: 23.0 Å

EMDB-9168:
Electron cryo-tomography and subtomogram averaging of microtubule triplet from procentriole
Method: EM (subtomogram averaging) / Resolution: 21.4 Å

EMDB-9169:
Electron cryo-tomography and subtomogram averaging of microtubule triplet from procentriole
Method: EM (subtomogram averaging) / Resolution: 22.3 Å

EMDB-9170:
Electron cryo-tomography and subtomogram averaging of microtubule triplet from procentriole
Method: EM (subtomogram averaging) / Resolution: 22.3 Å

EMDB-9171:
Electron cryo-tomography and subtomogram averaging of microtubule triplet from procentriole
Method: EM (subtomogram averaging) / Resolution: 23.1 Å

EMDB-9172:
Electron cryo-tomography and subtomogram averaging of microtubule triplet from procentriole
Method: EM (subtomogram averaging) / Resolution: 23.1 Å

EMDB-9173:
Electron cryo-tomography and subtomogram averaging of microtubule triplet from procentriole
Method: EM (subtomogram averaging) / Resolution: 23.1 Å

EMDB-9174:
Electron cryo-tomography and subtomogram averaging of microtubule triplet from procentriole
Method: EM (subtomogram averaging) / Resolution: 23.1 Å

Source
  • Chlamydomonas reinhardtii (plant)

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