EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of protein translocation by the Vps4-Vta1 AAA ATPase.
ジャーナル・号・ページ	Elife, Vol. 6, Year 2017
掲載日	2017年4月5日
著者	Nicole Monroe / Han Han / Peter S Shen / Wesley I Sundquist / Christopher P Hill /
PubMed 要旨	Many important cellular membrane fission reactions are driven by ESCRT pathways, which culminate in disassembly of ESCRT-III polymers by the AAA ATPase Vps4. We report a 4.3 Å resolution cryo-EM ...Many important cellular membrane fission reactions are driven by ESCRT pathways, which culminate in disassembly of ESCRT-III polymers by the AAA ATPase Vps4. We report a 4.3 Å resolution cryo-EM structure of the active Vps4 hexamer with its cofactor Vta1, ADP·BeF, and an ESCRT-III substrate peptide. Four Vps4 subunits form a helix whose interfaces are consistent with ATP binding, is stabilized by Vta1, and binds the substrate peptide. The fifth subunit approximately continues this helix but appears to be dissociating. The final Vps4 subunit completes a notched-washer configuration as if transitioning between the ends of the helix. We propose that ATP binding propagates growth at one end of the helix while hydrolysis promotes disassembly at the other end, so that Vps4 'walks' along ESCRT-III until it encounters the ordered N-terminal domain to destabilize the ESCRT-III lattice. This model may be generally applicable to other protein-translocating AAA ATPases.
リンク	Elife / PubMed:28379137 / PubMed Central
手法	EM (単粒子)
解像度	4.3 - 7.2 Å
構造データ	8549 5uie EMDB-8549, PDB-5uie: Vps4-Vta1 complex 手法: EM (単粒子) / 解像度: 5.7 Å EMDB-8550: Vps4-Vta1 complex, sharpened map 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-8551: Vps4-HCP hexamer 手法: EM (単粒子) / 解像度: 6.7 Å EMDB-8552: Vps4-Vta1 complex, VSL_A 手法: EM (単粒子) / 解像度: 5.4 Å EMDB-8553: Vps4-Vta1 complex, VSL_B 手法: EM (単粒子) / 解像度: 6.7 Å EMDB-8554: Vps4-Vta1 complex, VSL_C 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-8555: Vps4-Vta1 complex, VSL_D 手法: EM (単粒子) / 解像度: 6.9 Å EMDB-8556: Vps4-Vta1 complex, VSL_E 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-8557: Vps4-Vta1 complex, VSL_F 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-8570: Vps4-Vta1 complex, State 3 of subunitF 手法: EM (単粒子) / 解像度: 6.9 Å EMDB-8571: Vps4-Vta1 complex, State 2 of subunitF 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-8572: Vps4-Vta1 complex, State 1 of subunitF 手法: EM (単粒子) / 解像度: 6.9 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae (パン酵母)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / Vps4 / ESCRT (ESCRT) / Vta1 / AAA ATPase