Movie
Controller
Structure viewers
About Yorodumi Papers
+Search query
-Structure paper
| Title | Alignment algorithms and per-particle CTF correction for single particle cryo-electron tomography. |
|---|---|
| Journal, issue, pages | J Struct Biol, Vol. 194, Issue 3, Page 383-394, Year 2016 |
| Publish date | Mar 22, 2016 |
 Authors | Jesús G Galaz-Montoya / Corey W Hecksel / Philip R Baldwin / Eryu Wang / Scott C Weaver / Michael F Schmid / Steven J Ludtke / Wah Chiu /  |
| PubMed Abstract | Single particle cryo-electron tomography (cryoSPT) extracts features from cryo-electron tomograms, followed by 3D classification, alignment and averaging to generate improved 3D density maps of such ...Single particle cryo-electron tomography (cryoSPT) extracts features from cryo-electron tomograms, followed by 3D classification, alignment and averaging to generate improved 3D density maps of such features. Robust methods to correct for the contrast transfer function (CTF) of the electron microscope are necessary for cryoSPT to reach its resolution potential. Many factors can make CTF correction for cryoSPT challenging, such as lack of eucentricity of the specimen stage, inherent low dose per image, specimen charging, beam-induced specimen motions, and defocus gradients resulting both from specimen tilting and from unpredictable ice thickness variations. Current CTF correction methods for cryoET make at least one of the following assumptions: that the defocus at the center of the image is the same across the images of a tiltseries, that the particles all lie at the same Z-height in the embedding ice, and/or that the specimen, the cryo-electron microscopy (cryoEM) grid and/or the carbon support are flat. These experimental conditions are not always met. We have developed a CTF correction algorithm for cryoSPT without making any of the aforementioned assumptions. We also introduce speed and accuracy improvements and a higher degree of automation to the subtomogram averaging algorithms available in EMAN2. Using motion-corrected images of isolated virus particles as a benchmark specimen, recorded with a DE20 direct detection camera, we show that our CTF correction and subtomogram alignment routines can yield subtomogram averages close to 4/5 Nyquist frequency of the detector under our experimental conditions. |
 External links | J Struct Biol / PubMed:27016284 / PubMed Central |
| Methods | EM (subtomogram averaging) |
| Resolution | 13.0 Å |
| Structure data |  EMDB-8071: |
