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TitleRole of bacteriophage T4 baseplate in regulating assembly and infection.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 113, Issue 10, Page 2654-2659, Year 2016
Publish dateMar 8, 2016
AuthorsMoh Lan Yap / Thomas Klose / Fumio Arisaka / Jeffrey A Speir / David Veesler / Andrei Fokine / Michael G Rossmann /
PubMed AbstractBacteriophage T4 consists of a head for protecting its genome and a sheathed tail for inserting its genome into a host. The tail terminates with a multiprotein baseplate that changes its conformation ...Bacteriophage T4 consists of a head for protecting its genome and a sheathed tail for inserting its genome into a host. The tail terminates with a multiprotein baseplate that changes its conformation from a "high-energy" dome-shaped to a "low-energy" star-shaped structure during infection. Although these two structures represent different minima in the total energy landscape of the baseplate assembly, as the dome-shaped structure readily changes to the star-shaped structure when the virus infects a host bacterium, the dome-shaped structure must have more energy than the star-shaped structure. Here we describe the electron microscopy structure of a 3.3-MDa in vitro-assembled star-shaped baseplate with a resolution of 3.8 Å. This structure, together with other genetic and structural data, shows why the high-energy baseplate is formed in the presence of the central hub and how the baseplate changes to the low-energy structure, via two steps during infection. Thus, the presence of the central hub is required to initiate the assembly of metastable, high-energy structures. If the high-energy structure is formed and stabilized faster than the low-energy structure, there will be insufficient components to assemble the low-energy structure.
External linksProc Natl Acad Sci U S A / PubMed:26929357 / PubMed Central
MethodsEM (single particle)
Resolution3.8 Å
Structure data

8064

5hx2

EMDB-8064, PDB-5hx2:
In vitro assembled star-shaped hubless T4 baseplate
Method: EM (single particle) / Resolution: 3.8 Å

Source
  • enterobacteria phage t4 (virus)
KeywordsVIRAL PROTEIN / T4 / baseplate / complex

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