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-Structure paper
Title | Insights into centriole geometry revealed by cryotomography of doublet and triplet centrioles. |
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Journal, issue, pages | Elife, Vol. 7, Year 2018 |
Publish date | Aug 6, 2018 |
Authors | Garrett A Greenan / Bettina Keszthelyi / Ronald D Vale / David A Agard / |
PubMed Abstract | Centrioles are cylindrical assemblies comprised of 9 singlet, doublet, or triplet microtubules, essential for the formation of motile and sensory cilia. While the structure of the cilium is being ...Centrioles are cylindrical assemblies comprised of 9 singlet, doublet, or triplet microtubules, essential for the formation of motile and sensory cilia. While the structure of the cilium is being defined at increasing resolution, centriolar structure remains poorly understood. Here, we used electron cryo-tomography to determine the structure of mammalian (triplet) and (doublet) centrioles. Mammalian centrioles have two distinct domains: a 200 nm proximal core region connected by A-C linkers, and a distal domain where the C-tubule is incomplete and a pair of novel linkages stabilize the assembly producing a geometry more closely resembling the ciliary axoneme. centrioles resemble the mammalian core, but with their doublet microtubules linked through the A tubules. The commonality of core-region length, and the abrupt transition in mammalian centrioles, suggests a conserved length-setting mechanism. The unexpected linker diversity suggests how unique centriolar architectures arise in different tissues and organisms. |
External links | Elife / PubMed:30080137 / PubMed Central |
Methods | EM (subtomogram averaging) / EM (tomography) |
Resolution | 25.0 - 30.0 Å |
Structure data | EMDB-7775: EMDB-7776: EMDB-7777: EMDB-7778: EMDB-7779: |
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