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TitleCharacterization Standard for Cryo-electron Tomography.
Journal, issue, pagesbioRxiv, Year 2026
Publish dateMay 22, 2026
AuthorsMallak Ali / Joshua Hutchings / Tahiti Dutta / Nikki Jean / Garret Greenan / Elizabeth A Montabana / Jonathan Schwartz / M G Finn / Matthias Haury / David A Agard / Bridget Carragher / Mykhailo Kopylov / Mohammadreza Paraan /
PubMed AbstractStandardized biological specimens are essential for optimizing cryoEM workflows and benchmarking instrument performance. While apoferritin fulfills this role for single-particle analysis, no ...Standardized biological specimens are essential for optimizing cryoEM workflows and benchmarking instrument performance. While apoferritin fulfills this role for single-particle analysis, no equivalent exists for cryo-electron tomography. Ribosomes are frequently used but require large datasets due to C1 symmetry and structural heterogeneity, limiting rapid optimization and standardized comparison of workflows. Here, we present PP7 virus-like particles (VLPs) overexpressed in as a scalable in situ benchmark. VLPs have high orders of symmetry enabling rapid, high-resolution validation of tomographic pipelines from minimal datasets, while their distinct structural features across low to high resolutions provide a practical resolution metric.
External linksbioRxiv / PubMed:42239035 / PubMed Central
MethodsEM (subtomogram averaging) / EM (single particle)
Resolution2.89 - 4.2 Å
Structure data

EMDB-77085: Characterization standard for in-situ cryo-electron tomography: structure of PP7 virus-like-particle in E. coli from plunge freezing (full dataset)
Method: EM (subtomogram averaging) / Resolution: 3.0 Å

EMDB-77086: Characterization standard for in-situ cryo-electron tomography: structure of PP7 virus-like-particle in E. coli from high-pressure freezing and FIB-milling (full dataset)
Method: EM (subtomogram averaging) / Resolution: 3.9 Å

EMDB-77087: Characterization standard for in-situ cryo-electron tomography: structure of PP7 virus-like-particle in E. coli from plunge freezing (partial dataset)
Method: EM (subtomogram averaging) / Resolution: 3.8 Å

EMDB-77088: Characterization standard for in-situ cryo-electron tomography: structure of PP7 virus-like-particle in E. coli from high-pressure freezing and FIB-milling (partial dataset)
Method: EM (subtomogram averaging) / Resolution: 4.2 Å

EMDB-77092: Characterization standard for in-situ cryo-electron tomography: structure of PP7 virus-like-particle in E. coli from plunge freezing by single particle analysis
Method: EM (single particle) / Resolution: 2.89 Å

Source
  • Escherichia coli (E. coli)

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