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TitlePIP activation of the cardiac I potassium channel.
Journal, issue, pagesRes Sq, Year 2025
Publish dateOct 21, 2025
AuthorsJianmin Cui / Lu Zhao / Xianjin Xu / Chenxi Cui / Rui Duan / Ali Kermani / Jingyi Shi / Lu Han / Ji Sun / Xiaoqin Zou /
PubMed AbstractThe I channel complex, composed of the voltage-gated potassium channel KCNQ1 and its regulatory subunit KCNE1, is essential for the termination of cardiac action potentials. The function of KCNQ1 and ...The I channel complex, composed of the voltage-gated potassium channel KCNQ1 and its regulatory subunit KCNE1, is essential for the termination of cardiac action potentials. The function of KCNQ1 and I requires PIP, and its depletion abolishes channel opening. Previous studies revealed that KCNQ1 adopts both bent and straight conformations and can bind two PIP molecules: one adjacent to VSD (V-PIP), and the other at the VSD-pore interface (C-PIP). Here we show that the two PIP perform essential yet distinct roles: V-PIP enables the bent-to-straight transition, whereas C-PIP mediates VSD-pore coupling and stabilizes the straight conformation. Structure-function analysis and molecular dynamic simulations show that VSD activation elevates the V-PIP site and weakens the CaM-VSD interaction, permitting the conformational shift from the bent, intermediate open (IO) state associated with KCNQ1 to the straight, I-exclusive activated open (AO) state, which is further stabilized by C-PIP. Leveraging this mechanism, we developed a compound CA1, which selectively targets the V-PIP site and modulates I channel activity without affecting KCNQ1, offering a novel and promising conceptional path for specific and safe antiarrhythmic therapeutics.
External linksRes Sq / PubMed:41282193 / PubMed Central
MethodsEM (single particle)
Resolution3.47 Å
Structure data

76332

12dk

EMDB-76332, PDB-12dk:
structure of human KCNQ1-CaM-PIP2 intermediate state
Method: EM (single particle) / Resolution: 3.47 Å

Chemicals

ChemComp-PT5:
[(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho / phospholipid*YM

ChemComp-CA:
Unknown entry

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / KCNQ1 / intermediate state / PIP2

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